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AB166913

Anti-Gemin 3 antibody [EPR11283]

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(1 Publication)

Rabbit Recombinant Monoclonal Gemin 3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DP103, GEMIN3, DDX20, Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (AB166913)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (AB166913)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (AB166913)

Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Gemin 3 antibody [EPR11283] (AB166913)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (AB166913)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Gemin 3 antibody [EPR11283] (AB166913)
  • WB

Unknown

Western blot - Anti-Gemin 3 antibody [EPR11283] (AB166913)

All lanes:

Western blot - Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11283

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gemin 3 also known as gemin3 is a protein that acts as an RNA helicase and plays an essential mechanical role in RNA processing and metabolism. It is part of the family of DEAD-box proteins and is highly conserved across species. The molecular mass of Gemin 3 is about 95 kDa. Expression of Gemin 3 occurs in various tissues with significant presence in the brain spinal cord and other neuronal tissues indicating its important role in neurological function.
Biological function summary

Gemin 3 forms part of the large multiprotein complex known as the survival of motor neuron (SMN) complex. This complex participates in the assembly of small nuclear ribonucleoproteins (snRNPs) which are fundamental components of the spliceosome. Gemin 3 contributes to spliceosome biogenesis and the management of RNA processing activities which are essential for normal cellular functions and gene expression regulation.

Pathways

Gemin 3 is critically involved in the SMN-mediated snRNP assembly pathway and also plays a role in the regulation of mRNA splicing. In these pathways Gemin 3 closely interacts with other proteins in the SMN complex such as Gemin 2 and Gemin 4 which are important for the stability and function of the entire snRNP assembly process. These interactions are necessary for efficient pre-mRNA splicing ensuring proper genetic information flow and expression.

Gemin 3 is directly linked to spinal muscular atrophy (SMA) a neurodegenerative disorder caused by mutations in the SMN1 gene affecting the SMN complex. When the SMN complex malfunctions due to inadequate cooperation between SMN and Gemin 3 it leads to defective snRNP assembly causing loss of motor neurons and muscle wasting characteristic of SMA. Additionally altered Gemin 3 function can be associated with certain other neurodegenerative conditions where RNA processing is impaired highlighting the protein's importance in maintaining neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
See full target information DDX20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2421915122 PubMed40294271

2025

Inhaled mRNA therapy for treatment of primary ciliary dyskinesia.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Hennig,Rumpa B Bhattacharjee,Ishita Agarwal,Ali Alfaifi,Jade E Casillas,Sofia Chavez,Daniella Ishimaru,David Liston,Sakya Mohapatra,Touhidul Molla,Suyog Pathare,Maninder S Sidhu,Peng Wang,Zechen Wang,T Noelle Lombana,Vladimir G Kharitonov,Jessica A Couch,David J Lockhart,Brandon A Wustman
View all publications
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Product promise

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