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AB249394

Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free

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Rabbit Recombinant Monoclonal Gemin 3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

DP103, GEMIN3, DDX20, Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)
  • WB

Unknown

Western blot - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (AB249394)

This data was developed using ab166913, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Gemin 3 antibody [EPR11283] (<a href='/en-us/products/primary-antibodies/gemin-3-antibody-epr11283-ab166913'>ab166913</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Predicted band size: 92 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11283

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249394 is the carrier-free version of ab166913.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gemin 3 also known as gemin3 is a protein that acts as an RNA helicase and plays an essential mechanical role in RNA processing and metabolism. It is part of the family of DEAD-box proteins and is highly conserved across species. The molecular mass of Gemin 3 is about 95 kDa. Expression of Gemin 3 occurs in various tissues with significant presence in the brain spinal cord and other neuronal tissues indicating its important role in neurological function.
Biological function summary

Gemin 3 forms part of the large multiprotein complex known as the survival of motor neuron (SMN) complex. This complex participates in the assembly of small nuclear ribonucleoproteins (snRNPs) which are fundamental components of the spliceosome. Gemin 3 contributes to spliceosome biogenesis and the management of RNA processing activities which are essential for normal cellular functions and gene expression regulation.

Pathways

Gemin 3 is critically involved in the SMN-mediated snRNP assembly pathway and also plays a role in the regulation of mRNA splicing. In these pathways Gemin 3 closely interacts with other proteins in the SMN complex such as Gemin 2 and Gemin 4 which are important for the stability and function of the entire snRNP assembly process. These interactions are necessary for efficient pre-mRNA splicing ensuring proper genetic information flow and expression.

Gemin 3 is directly linked to spinal muscular atrophy (SMA) a neurodegenerative disorder caused by mutations in the SMN1 gene affecting the SMN complex. When the SMN complex malfunctions due to inadequate cooperation between SMN and Gemin 3 it leads to defective snRNP assembly causing loss of motor neurons and muscle wasting characteristic of SMA. Additionally altered Gemin 3 function can be associated with certain other neurodegenerative conditions where RNA processing is impaired highlighting the protein's importance in maintaining neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
See full target information DDX20
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Product promise

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