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Rabbit Recombinant Monoclonal Gemin 5 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (AB251353), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (AB251353), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (AB251353), expandable thumbnail
  • Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (AB251353), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Gemin 5 antibody [EPR17841] - BSA and Azide free (AB251353), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:16857593, PubMed:18984161, PubMed:20513430, PubMed:33963192). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate (PubMed:18984161). Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN5 recognizes and delivers the small nuclear RNAs (snRNAs) to the SMN complex (PubMed:11714716, PubMed:16314521, PubMed:16857593, PubMed:19377484, PubMed:19750007, PubMed:20513430, PubMed:27834343, PubMed:27881600, PubMed:27881601). Binds to the 7-methylguanosine cap of RNA molecules (PubMed:19750007, PubMed:27834343, PubMed:27881600, PubMed:27881601, Ref.27). Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (PubMed:27507887).

Alternative names

Gem-associated protein 5, Gemin5, GEMIN5

Recommended products

Rabbit Recombinant Monoclonal Gemin 5 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17841
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251353 is the carrier-free version of Anti-Gemin 5 antibody [EPR17841] ab201691.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gemin 5 also known as GEMIN5 is a multi-functional RNA-binding protein functioning critically in several cellular mechanisms. It has a mass of approximately 170 kDa. Gemin 5 expresses in various tissues highlighting its diverse roles in cellular activities. One of its primary mechanical roles involves the recognition and binding of snRNA which is necessary for the spliceosomal snRNP assembly. It interacts with other proteins within the SMN complex to exert its function.

Biological function summary

Gemin 5 plays an important role in RNA processing pathways and is integral to the formation and functioning of the SMN complex. This complex involves several related proteins including Gemin 2 Gemin 3 and SMN1 and is essential for the biogenesis of small nuclear ribonucleoproteins (snRNPs). Gemin 5 possesses specific domains that allow the binding to snRNA facilitating the assembly of spliceosomal units which are critical for the splicing of pre-mRNA into mature mRNA.

Pathways

Gemin 5's involvement in spliceosomal snRNP assembly places it within the RNA processing and gene expression regulatory pathways. Its interaction with SMN1 and Gemin 3 positions it as an important component in these pathways specifically influencing the splicing pathway. The assembly of snRNPs and subsequent influencing of RNA splicing illustrate Gemin 5's role in maintaining gene expression fidelity which is pivotal for cellular function and homeostasis.

Associated diseases and disorders

Gemin 5's dysregulation or malfunction associates significantly with spinal muscular atrophy (SMA) and certain neurodegenerative conditions. This association mainly arises through its interaction with SMN1 which when mutated or deficient results in SMA due to impaired snRNP assembly and subsequent motor neuron degeneration. Additionally potential links exist with neurodegenerative disorders where RNA processing defects are implicated though the full scope of its involvement requires further research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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