Skip to main content

Mouse Monoclonal Geminin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMNN aa 100 to C-terminus.


Images

Western blot - Anti-Geminin antibody [1A8] (AB104306), expandable thumbnail
  • Western blot - Anti-Geminin antibody [1A8] (AB104306), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (AB104306), expandable thumbnail
  • Flow Cytometry - Anti-Geminin antibody [1A8] (AB104306), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (AB104306), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human GMNN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75496

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed:14993212, PubMed:20129055, PubMed:24064211, PubMed:9635433). It is degraded during the mitotic phase of the cell cycle (PubMed:14993212, PubMed:24064211, PubMed:9635433). Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed:14993212, PubMed:24064211, PubMed:9635433). Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (PubMed:20129055). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (PubMed:22615398).

Alternative names

Geminin, GMNN

Recommended products

Mouse Monoclonal Geminin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMNN aa 100 to C-terminus.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human GMNN aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75496
Carrier free
Yes
Clone number
1A8
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Geminin also referred to as GMNN is a protein that regulates DNA replication. It has approximately 25 kDa mass. Geminin inhibits DNA replication by preventing the loading of the mini-chromosome maintenance complex onto DNA during the cell cycle. This function ensures that DNA replication takes place only once per cell cycle. Geminin expresses mainly in tissues with high proliferative activity such as the developing nervous system and hematopoietic cells. Its interaction with other proteins plays a significant role in controlling cell cycle progression.

Biological function summary

In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.

Pathways

The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.

Associated diseases and disorders

Aberrations in Geminin activity can lead to severe conditions such as cancer. Its overexpression or misregulation has a link to various tumor types due to its effect on uncontrolled cell proliferation. Geminin's interaction with proteins like Paxillin and Cyclin-dependent kinases also relates to cancer progression as these interactions may involve changes in cellular adhesion and cycle checkpoints. Therefore understanding Geminin's role offers valuable insight for potential therapeutic targets in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Western blot - Anti-Geminin antibody [1A8] (ab104306)

    The tagged immunogen has a predicted MWt of 37 kDa.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Geminin antibody [1A8] (ab104306) at 5 µg/mL

    All lanes: Immunogen at 0.2 µg

    Predicted band size: 24 kDa

  • Western blot - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Western blot - Anti-Geminin antibody [1A8] (ab104306)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Geminin antibody [1A8] (ab104306) at 5 µg/mL

    Lane 1: Geminin transfected 293T cell lysate at 25 µg

    Lane 2: Non-transfected 293T cell lysate at 25 µg

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail
    This image is courtesy of a customer review submitted by Melody Jimenez

    Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (ab104306)

    Immunofluorescence analysis of Caki-2 (Clear cell renal carcinoma) cells, staining Geminin with ab104306.

    Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at 25oC. Primary antibody was concentrated using Antibody Concentration And Clean-Up Kit ab102778 Antibody Concentration Kit and conjugated to Rhodamine using ab102915 EasyLink Rhodamine Conjugation Kit. Samples were incubated with conjugated primary antibody (20 μg/ml in diluent) for 2 hours at 25oC.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Flow Cytometry - Anti-Geminin antibody [1A8] (ab104306)

    Overlay histogram showing HeLa cells stained with ab104306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104306, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (ab104306)

    ab104306, at 3 μg/ml, staining Geminin in formalin-fixed paraffin-embedded Human lymph node by Immunohistochemistry.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (ab104306)

    ab104306, at 1μg/ml, staining Geminin in formalin-fixed paraffin-embedded Human placenta by Immunohistochemistry.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (ab104306), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (ab104306)

    ab104306, at 10 μg/ml, staining Geminin in HeLa cells by Immunofluorescence.

    This image was generated using the ascites version of the product.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com