AB175799

Anti-Geminin antibody

Name: Anti-Geminin antibody (ab175799) | Abcam
Brand: Abcam Limited
SKU: AB175799
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal Geminin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GMNN.

View Alternative Names

Geminin, GMNN

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (AB175799)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (AB175799)

Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling Geminin with ab175799 at 1 : 1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Middle - 10X, Right - 20X.

Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody (AB175799)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody (AB175799)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Geminin with ab175799 at 1 : 1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Geminin, CyclinB, DAPI, Merge.

Supplier Data

Immunoprecipitation - Anti-Geminin antibody (AB175799)

Immunoprecipitation analysis of U2OS cells labelling Geminin with ab175799. Protein A beads were crosslinked to the serum.

All lanes:

Immunoprecipitation - Anti-Geminin antibody (ab175799)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Geminin antibody (AB175799)

All lanes:

Western blot - Anti-Geminin antibody (ab175799)

Lane 1:

U2OS (Control si)

Lane 2:

U2OS (Geminin si)

Lane 3:

NIH 3T3 cell lysate at 40 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GMNN.

O75496

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Water

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Geminin also referred to as GMNN is a protein that regulates DNA replication. It has approximately 25 kDa mass. Geminin inhibits DNA replication by preventing the loading of the mini-chromosome maintenance complex onto DNA during the cell cycle. This function ensures that DNA replication takes place only once per cell cycle. Geminin expresses mainly in tissues with high proliferative activity such as the developing nervous system and hematopoietic cells. Its interaction with other proteins plays a significant role in controlling cell cycle progression.

Biological function summary

In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.

Pathways

The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.

Aberrations in Geminin activity can lead to severe conditions such as cancer. Its overexpression or misregulation has a link to various tumor types due to its effect on uncontrolled cell proliferation. Geminin's interaction with proteins like Paxillin and Cyclin-dependent kinases also relates to cancer progression as these interactions may involve changes in cellular adhesion and cycle checkpoints. Therefore understanding Geminin's role offers valuable insight for potential therapeutic targets in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed : 14993212, PubMed : 20129055, PubMed : 24064211, PubMed : 9635433). It is degraded during the mitotic phase of the cell cycle (PubMed : 14993212, PubMed : 24064211, PubMed : 9635433). Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed : 14993212, PubMed : 24064211, PubMed : 9635433). Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (PubMed : 20129055). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (PubMed : 22615398).

See full target information GMNN

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:781 PubMed35918603

2022

Gonadal sex patterns p21-induced cellular senescence in mouse and human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Broestl,Nicole M Warrington,Lucia Grandison,Tamara Abou-Antoun,Olivia Tung,Saraswati Shenoy,Miranda M Tallman,Gina Rhee,Wei Yang,Jasmin Sponagel,Lihua Yang,Najla Kfoury-Beaumont,Cameron M Hill,Sulaiman A Qanni,Diane D Mao,Albert H Kim,Sheila A Stewart,Monica Venere,Jingqin Luo,Joshua B Rubin

Experimental cell research 408:112880 PubMed34655601

2021

Identification and characterization of distinct cell cycle stages in cardiomyocytes using the FUCCI transgenic system.

Applications

ICC

Species

Mouse

Marion Baniol,Francesca Murganti,Agata Smialowska,Joni Panula,Enikő Lázár,Viveka Brockman,Sarantis Giatrellis,Wouter Derks,Olaf Bergmann

eLife 10: PubMed34009124

2021

Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree.

Applications

Unspecified application

Species

Mouse

Audrey M Hendley,Arjun A Rao,Laura Leonhardt,Sudipta Ashe,Jennifer A Smith,Simone Giacometti,Xianlu L Peng,Honglin Jiang,David I Berrios,Mathias Pawlak,Lucia Y Li,Jonghyun Lee,Eric A Collisson,Mark S Anderson,Gabriela K Fragiadakis,Jen Jen Yeh,Chun Jimmie Ye,Grace E Kim,Valerie M Weaver,Matthias Hebrok

Nature 547:61-67 PubMed28682332

2017

Cell-cycle dynamics of chromosomal organization at single-cell resolution.

Applications

Flow Cyt

Species

Mouse

Takashi Nagano,Yaniv Lubling,Csilla Várnai,Carmel Dudley,Wing Leung,Yael Baran,Netta Mendelson Cohen,Steven Wingett,Peter Fraser,Amos Tanay

Oncogene 36:4802-4809 PubMed28288134

2017

DUB3 and USP7 de-ubiquitinating enzymes control replication inhibitor Geminin: molecular characterization and associations with breast cancer.

Applications

Species

Human

S Hernández-Pérez,E Cabrera,E Salido,M Lim,L Reid,S R Lakhani,K K Khanna,J M Saunus,R Freire

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