Mouse Monoclonal Geminin antibody. Carrier free. Suitable for Protein Array, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GMNN.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219), expandable thumbnail](https://content.abcam.com/products/images/geminin-antibody-cptc-gmnn-1-bsa-and-azide-free-ab268219--immunocytochemistry-immunofluorescence-img108051.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)")
![Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219), expandable thumbnail](https://content.abcam.com/products/images/geminin-antibody-cptc-gmnn-1-bsa-and-azide-free-ab268219--protein-array-img139726.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GMNN. Database link O75496
Reactivity data
Select an application| Protein Array | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Recombinant full length protein - Human | Tested | Not recommended |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
5 products for Alternative Product
Target data
Function
Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed:14993212, PubMed:20129055, PubMed:24064211, PubMed:9635433). It is degraded during the mitotic phase of the cell cycle (PubMed:14993212, PubMed:24064211, PubMed:9635433). Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed:14993212, PubMed:24064211, PubMed:9635433). Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (PubMed:20129055). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (PubMed:22615398).
Alternative names
Geminin, GMNN
Recommended products
Mouse Monoclonal Geminin antibody. Carrier free. Suitable for Protein Array, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GMNN.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GMNN. Database link O75496
- Carrier free
- Yes
- Clone number
- CPTC-GMNN-1
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor concentrate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Geminin also referred to as GMNN is a protein that regulates DNA replication. It has approximately 25 kDa mass. Geminin inhibits DNA replication by preventing the loading of the mini-chromosome maintenance complex onto DNA during the cell cycle. This function ensures that DNA replication takes place only once per cell cycle. Geminin expresses mainly in tissues with high proliferative activity such as the developing nervous system and hematopoietic cells. Its interaction with other proteins plays a significant role in controlling cell cycle progression.
- Biological function summary
In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.
- Pathways
The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.
- Associated diseases and disorders
Aberrations in Geminin activity can lead to severe conditions such as cancer. Its overexpression or misregulation has a link to various tumor types due to its effect on uncontrolled cell proliferation. Geminin's interaction with proteins like Paxillin and Cyclin-dependent kinases also relates to cancer progression as these interactions may involve changes in cellular adhesion and cycle checkpoints. Therefore understanding Geminin's role offers valuable insight for potential therapeutic targets in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219), expandable thumbnail](https://content.abcam.com/products/images/geminin-antibody-cptc-gmnn-1-bsa-and-azide-free-ab268219--immunocytochemistry-immunofluorescence-img108051.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219)")
Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219)
Immunocytochemistry/ Immunofluorescence staining of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Geminin with Anti-Geminin antibody [CPTC-GMNN-1] ab268098 at 2 μg/ml followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Geminin antibody [CPTC-GMNN-1] ab268098).
![Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219), expandable thumbnail](https://content.abcam.com/products/images/geminin-antibody-cptc-gmnn-1-bsa-and-azide-free-ab268219--protein-array-img139726.jpg "Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219)")
Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (ab268219)
Protein Array containing more than 19,000 full-length human proteins using Anti-Geminin antibody [CPTC-GMNN-1] ab268098.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Geminin antibody [CPTC-GMNN-1] ab268098).
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