Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free
Be the first to review this product! Submit a review
|
(0 Publication)
Mouse Monoclonal Geminin antibody. Carrier free. Suitable for Protein Array, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GMNN.
View Alternative Names
Geminin, GMNN
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)
Immunocytochemistry/ Immunofluorescence staining of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Geminin with ab268098 at 2 μg/ml followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268098).
- Protein Array
Unknown
Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)
Protein Array containing more than 19,000 full-length human proteins using ab268098.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268098).
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.
Pathways
The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com