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AB268219

Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free

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Mouse Monoclonal Geminin antibody. Carrier free. Suitable for Protein Array, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GMNN.

View Alternative Names

Geminin, GMNN

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)

Immunocytochemistry/ Immunofluorescence staining of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Geminin with ab268098 at 2 μg/ml followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268098).

Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)
  • Protein Array

Unknown

Protein Array - Anti-Geminin antibody [CPTC-GMNN-1] - BSA and Azide free (AB268219)

Protein Array containing more than 19,000 full-length human proteins using ab268098.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268098).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CPTC-GMNN-1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GMNN.

O75496

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor concentrate
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Geminin also referred to as GMNN is a protein that regulates DNA replication. It has approximately 25 kDa mass. Geminin inhibits DNA replication by preventing the loading of the mini-chromosome maintenance complex onto DNA during the cell cycle. This function ensures that DNA replication takes place only once per cell cycle. Geminin expresses mainly in tissues with high proliferative activity such as the developing nervous system and hematopoietic cells. Its interaction with other proteins plays a significant role in controlling cell cycle progression.
Biological function summary

In a cellular context Geminin forms part of a complex with the nuclear protein Cdt1. This complex is essential in the regulation of the cell cycle's S phase. Geminin's function restricts DNA replication by binding to Cdt1 and stopping its activity which is necessary during the G1/S phase transition. By doing so it preserves the integrity of genetic information and prevents re-replication. Geminin's inhibition remains strict until it is degraded during mitosis allowing Cdt1 to resume its function in the following cell cycle.

Pathways

The regulation of Geminin is important to maintaining proper DNA replication and cell cycle integrity. Geminin plays a role in the cell cycle control pathway particularly during the G1 to S phase transition. Additionally it interfaces with other proteins like Paxillin which is involved in cell adhesion and movement. Although not directly linked in a pathway Paxillin's role in regulating cellular dynamics can influence cell cycle processes in which Geminin is involved. Geminin also serves as a K blocker relevant in maintaining genetic stability.

Aberrations in Geminin activity can lead to severe conditions such as cancer. Its overexpression or misregulation has a link to various tumor types due to its effect on uncontrolled cell proliferation. Geminin's interaction with proteins like Paxillin and Cyclin-dependent kinases also relates to cancer progression as these interactions may involve changes in cellular adhesion and cycle checkpoints. Therefore understanding Geminin's role offers valuable insight for potential therapeutic targets in oncology.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed : 14993212, PubMed : 20129055, PubMed : 24064211, PubMed : 9635433). It is degraded during the mitotic phase of the cell cycle (PubMed : 14993212, PubMed : 24064211, PubMed : 9635433). Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle (PubMed : 14993212, PubMed : 24064211, PubMed : 9635433). Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing (PubMed : 20129055). Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (PubMed : 22615398).
See full target information GMNN

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