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AB247277

Anti-General Receptor for phosphoinositides 1/GRASP antibody [EP394Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal General Receptor for phosphoinositides 1/GRASP antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GRASP, TAMALIN, Protein TAMALIN, General receptor for phosphoinositides 1-associated scaffold protein, GRP1-associated scaffold protein

1 Images
Western blot - Anti-General Receptor for phosphoinositides 1/GRASP antibody [EP394Y] - BSA and Azide free (AB247277)
  • WB

Unknown

Western blot - Anti-General Receptor for phosphoinositides 1/GRASP antibody [EP394Y] - BSA and Azide free (AB247277)

This data was developed using ab33886, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-General Receptor for phosphoinositides 1/GRASP antibody [EP394Y] (<a href='/en-us/products/primary-antibodies/general-receptor-for-phosphoinositides-1-grasp-antibody-ep394y-ab33886'>ab33886</a>) at 1/500 dilution

All lanes:

Human brain lysate

Predicted band size: 43 kDa

Observed band size: 45 kDa

false

  • Unconjugated

    Anti-General Receptor for phosphoinositides 1/GRASP antibody [EP394Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP394Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab247277 is the carrier-free version of ab33886.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The General Receptor for Phosphoinositides 1 (GRASP) also known as GRP1 is a protein that functions as a phosphoinositide-binding protein and regulates cellular membrane dynamics. It has a mass of approximately 97 kDa. GRASP is expressed in various tissues with significant presence in the brain and immune cells. Mechanically GRASP interacts with phosphoinositides on cellular membranes which assists in membrane trafficking and signaling.
Biological function summary

GRASP has a role in modulating intracellular transport processes and is critical in endosomal trafficking. It operates as part of a larger complex that regulates the recycling of membranes and proteins within cells. GRASP aids in ensuring that the proteins and membranes reach their intended cellular destinations maintaining cellular homeostasis.

Pathways

The protein is an important participant in the phosphoinositide 3-kinase (PI3K) signaling pathway an important pathway in cell growth proliferation and survival. GRASP interacts with proteins such as ARF6 influencing membrane dynamics and cellular signaling. Involved in these pathways GRASP contributes to proper cellular responses to extracellular signals and impacts various downstream biological effects.

GRASP plays a critical role in neurological disorders and immune system irregularities. Disruptions in GRASP function can be associated with conditions like Parkinson's disease and systemic lupus erythematosus (SLE). In Parkinson's GRASP interacts with proteins like LRRK2 suggesting a link between phosphoinositide signaling and neurodegenerative pathogenesis. In the immune context alterations in GRASP function seem connected to immune cell signaling irregularities influencing SLE development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.
See full target information TAMALIN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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