Sheep Polyclonal Gentamicin antibody. Suitable for ELISA, cELISA and reacts with Chemical samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Gentamicin.
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.88% Sodium chloride
| ELISA | cELISA | |
|---|---|---|
| Chemical | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes 10ng/ml Gentamycin produces 89% inhibition in a competitive ELISA employing ab123989. |
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Gentamycin
Sheep Polyclonal Gentamicin antibody. Suitable for ELISA, cELISA and reacts with Chemical samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Gentamicin.
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.88% Sodium chloride
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Gentamicin is not a typical biological target but rather an aminoglycoside antibiotic used in targeting bacteria. It does not possess a protein structure with a mass as it is a compound. Gentamicin acts mechanically by binding to the 30S subunit of bacterial ribosomes causing a misreading of mRNA and disrupting protein synthesis. It mainly targets gram-negative bacteria but also shows activity against some gram-positive bacteria. Gentamicin is typically administered in settings like hospitals for treating severe infections.
The main activity of this antibiotic disrupts the normal function of the bacterial ribosome. It is not a part of a complex but interacts with the ribosome complex consisting of ribosomal RNA and various ribosomal proteins. By binding to the 30S subunit gentamicin causes errors in the translation process preventing the bacteria from synthesizing necessary proteins needed for growth and proliferation.
Disruptions caused by gentamicin impact the bacterial translation pathway significantly. It affects the elongation cycle of protein synthesis where aminoacyl-tRNA enters the ribosomal A site. The faulty incorporation of amino acids due to gentamicin's effect hinders the elongation pathway leading to nonfunctional proteins and bacterial death. This process relates components like other aminoglycoside antibiotics such as amikacin which function similarly.
Severe bacterial infections often require gentamicin intervention. It is particularly relevant in treating infections caused by Pseudomonas aeruginosa and those leading to conditions such as sepsis and complicated urinary tract infections. Proteins involved in bacterial virulence and resistance such as efflux pumps are associated with how certain bacteria may resist gentamicin action impacting its effectiveness in these diseases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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