AB185930

Anti-Gephyrin antibody

Name: Anti-Gephyrin antibody (ab185930) | Abcam
Brand: Abcam Limited
SKU: AB185930
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Gephyrin antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPHN aa 650 to C-terminus.

View Alternative Names

GPH, KIAA1385, GPHN, Gephyrin

1 Images

Supplier Data

Western blot - Anti-Gephyrin antibody (AB185930)

All lanes:

Western blot - Anti-Gephyrin antibody (ab185930) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 80 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human GPHN aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NQX3

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.

Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.

See full target information GPHN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Epilepsia 59:2061-2074 PubMed30324621

2018

γ-Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Éric Samarut,Amrutha Swaminathan,Raphaëlle Riché,Meijiang Liao,Rahma Hassan-Abdi,Solène Renault,Marc Allard,Liselotte Dufour,Patrick Cossette,Nadia Soussi-Yanicostas,Pierre Drapeau

Current biology : CB 28:1924-1937.e5 PubMed29861134

2018

Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development.

Applications

Unspecified application

Species

Unspecified reactive species

Amrutha Swaminathan,Rahma Hassan-Abdi,Solène Renault,Aleksandra Siekierska,Raphaëlle Riché,Meijiang Liao,Peter A M de Witte,Constantin Yanicostas,Nadia Soussi-Yanicostas,Pierre Drapeau,Éric Samarut

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Gephyrin antibody

Western blot - Anti-Gephyrin antibody (AB185930)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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