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AB228674

Anti-Gephyrin antibody

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(1 Publication)

Rabbit Polyclonal Gephyrin antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GPHN.

View Alternative Names

GPH, KIAA1385, GPHN, Gephyrin

7 Images
Immunoprecipitation - Anti-Gephyrin antibody (AB228674)
  • IP

Supplier Data

Immunoprecipitation - Anti-Gephyrin antibody (AB228674)

Gephyrin was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 μg ab228674. Western blot was performed from the immunoprecipitate using ab228674.

Lane 2 : Control IgG IP in A431 whole cell extract.

Lane 1 : ab228674 IP in A431 whole cell extract.

All lanes:

Immunoprecipitation - Anti-Gephyrin antibody (ab228674)

Predicted band size: 80 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody (AB228674)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody (AB228674)

Paraffin-embedded mouse brain tissue stained for Gephyrin with ab228674 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody (AB228674)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody (AB228674)

DIV10 rat E18 primary hippocampal neurons stained for Gephyrin (green) using ab228674 (1 : 500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500 (red). Fluoroshield with DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-Gephyrin antibody (AB228674)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Gephyrin antibody (AB228674)

Frozen sectioned E13.5 rat brain tissue stained for Gephyrin with ab228674 at 1/250 dilution (green) in immunohistochemical analysis.

Beta Tubulin 3/ TUJ1, a mature neuron marker, is detected with a beta Tubulin 3/ TUJ1 antibody (red). Nuclear counterstain : Fluoroshield with DAPI (blue).

Western blot - Anti-Gephyrin antibody (AB228674)
  • WB

Supplier Data

Western blot - Anti-Gephyrin antibody (AB228674)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Gephyrin antibody (ab228674) at 1/3000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extracts at 30 µg

Lane 3:

IMR32 (human neuroblast cell line) whole cell extracts at 30 µg

Lane 4:

SK-N-AS (human neuroblastoma cell line) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

true

Western blot - Anti-Gephyrin antibody (AB228674)
  • WB

Supplier Data

Western blot - Anti-Gephyrin antibody (AB228674)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Gephyrin antibody (ab228674) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

JC (mouse mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 80 kDa

true

Western blot - Anti-Gephyrin antibody (AB228674)
  • WB

Supplier Data

Western blot - Anti-Gephyrin antibody (AB228674)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Gephyrin antibody (ab228674) at 1/5000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, IHC-Fr, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human GPHN. The exact immunogen used to generate this antibody is proprietary information.

Q9NQX3

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.
Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
See full target information GPHN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40141229

2025

mTOR-Mediated Autophagy Regulates Cadmium-Induced Kidney Injury via Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Hu,Kui Wang,Jie Xu,Guohuan Wan,Yiyi Zhao,Yajing Chen,Kangfeng Jiang,Xiaobing Li
View all publications
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