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AB181382

Anti-Gephyrin antibody [EPR12650]

5

(3 Reviews)

|

(6 Publications)

Rabbit Recombinant Monoclonal Gephyrin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

GPH, KIAA1385, GPHN, Gephyrin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] (AB181382)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] (AB181382)

Immunohistochemical analysis of parafiin embedded human brain tissue using ab181382 (unpurified).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] (AB181382)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] (AB181382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Gephyrin with purified ab181382 at 1/100 dilution (1.62 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)
  • WB

Lab

Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181382 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab181382 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. ab181382 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Gephyrin antibody [EPR12650] (ab181382) at 1 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

GPHN (Gephyrin) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

MCF7 whole cell lysate at 20 µg

Lane 4:

U2OS whole cell lysate at 20 µg

Predicted band size: 80 kDa

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Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)
  • WB

Supplier Data

Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)

All lanes:

Western blot - Anti-Gephyrin antibody [EPR12650] (ab181382) at 1/1000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 80 kDa

false

Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)
  • WB

Lab

Western blot - Anti-Gephyrin antibody [EPR12650] (AB181382)

All lanes:

Western blot - Anti-Gephyrin antibody [EPR12650] (ab181382) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 93 kDa

false

  • Carrier free

    Anti-Gephyrin antibody [EPR12650] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12650

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.
Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
See full target information GPHN
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8747 PubMed41034202

2025

Cassini: streamlined and scalable method for in situ profiling of RNA and protein.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Lapique,Michael Taewoo Kim,Nicholas Thom,Naeem M Nadaf,Juan Pineda,Evan Z Macosko

Journal of extracellular vesicles 13:e12439 PubMed38647111

2024

Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Divya T Chemparathy,Sudipta Ray,Chase Ochs,Natasha Ferguson,Dinesh Y Gawande,Shashank M Dravid,Shannon Callen,Susmita Sil,Shilpa Buch

IUBMB life 76:548-562 PubMed38390757

2024

The complex relationship between late-onset caloric restriction and synaptic plasticity in aged Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Milica Prvulovic,Srdjan Sokanovic,Valentina Simeunovic,Andjela Vukojevic,Milena Jovic,Smilja Todorovic,Aleksandra Mladenovic

International journal of molecular sciences 23: PubMed36233065

2022

Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yaiza M Arenas,Mar Martínez-García,Marta Llansola,Vicente Felipo

Extracellular vesicles and circulating nucleic acids 3:133-149 PubMed36812097

2022

HIV-1 Tat induced microglial EVs leads to neuronal synaptodendritic injury: microglia-neuron cross-talk in NeuroHIV.

Applications

Unspecified application

Species

Unspecified reactive species

Muthukumar Kannan,Seema Singh,Divya T Chemparathy,Abiola A Oladapo,Dinesh Y Gawande,Shashank M Dravid,Shilpa Buch,Susmita Sil

Frontiers in neuroscience 12:985 PubMed30670942

2019

Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Lizarbe,Ana Francisca Soares,Sara Larsson,João M N Duarte
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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