Rabbit Recombinant Monoclonal Gephyrin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171013.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503)")
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img181314.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503)")
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img176251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503)")
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img183503.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (AB250503)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Rat | Expected | Tested | Not recommended |
| Chicken | Predicted | Predicted | Not recommended |
| Cow | Predicted | Predicted | Not recommended |
| Dog | Predicted | Predicted | Not recommended |
| Pig | Predicted | Predicted | Not recommended |
| Rhesus monkey | Predicted | Predicted | Not recommended |
| Xenopus tropicalis | Predicted | Predicted | Not recommended |
| Zebrafish | Predicted | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Chicken, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed:25025157, PubMed:26613940). Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
Alternative names
GPH, KIAA1385, GPHN, Gephyrin
Recommended products
Rabbit Recombinant Monoclonal Gephyrin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12650
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250503 is the carrier-free version of Anti-Gephyrin antibody [EPR12650] ab181382.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.
- Biological function summary
Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.
- Pathways
Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.
- Associated diseases and disorders
Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171013.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)
This data was developed using Anti-Gephyrin antibody [EPR12650] ab181382, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of parafiin embedded human brain tissue using Anti-Gephyrin antibody [EPR12650] ab181382 (unpurified).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemical analysis of parafiin embedded human brain tissue using Anti-Gephyrin antibody [EPR12650] ab181382 (unpurified).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Gephyrin with purified Anti-Gephyrin antibody [EPR12650] ab181382 at 1/100 dilution (1.62 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Gephyrin antibody [EPR12650] ab181382)
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img181314.jpg "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)")
Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)
This data was developed using Anti-Gephyrin antibody [EPR12650] ab181382, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503) at 1/1000 dilution
Lane 1: Fetal brain tissue lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: SH-SY5Y cell lysate at 10 µg
Predicted band size: 80 kDa
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img176251.jpg "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)")
Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)
This data was developed using Anti-Gephyrin antibody [EPR12650] ab181382, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg
Lane 2: Mouse heart lysates at 20 µg
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg
Lane 4: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 93 kDa
![Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503), expandable thumbnail](https://content.abcam.com/products/images/gephyrin-antibody-epr12650-bsa-and-azide-free-ab250503--western-blot-img183503.jpg "Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)")
Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503)
This data was developed using Anti-Gephyrin antibody [EPR12650] ab181382, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Gephyrin antibody [EPR12650] ab181382 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Gephyrin antibody [EPR12650] ab181382 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. Anti-Gephyrin antibody [EPR12650] ab181382 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Gephyrin antibody [EPR12650] - BSA and Azide free (ab250503) at 1 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: GPHN (Gephyrin) knockout HAP1 whole cell lysate at 20 µg
Lane 3: MCF7 whole cell lysate at 20 µg
Lane 4: U2OS whole cell lysate at 20 µg
Predicted band size: 80 kDa
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