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Rabbit Recombinant Monoclonal Gephyrin antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (AB177154), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12651(B)] (AB177154), expandable thumbnail
  • Western blot - Anti-Gephyrin antibody [EPR12651(B)] (AB177154), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Gephyrin antibody [EPR12651(B)] (AB177154), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (AB177154), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Expected
Tested
Not recommended
Tested
Expected
Rat
Expected
Tested
Not recommended
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Species
Mouse
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed:25025157, PubMed:26613940). Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.

Alternative names

GPH, KIAA1385, GPHN, Gephyrin

Recommended products

Rabbit Recombinant Monoclonal Gephyrin antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12651(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.

Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Associated diseases and disorders

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Gephyrin with ab177154 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Gephyrin with ab177154 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    Lanes 1 - 4: Merged signal (red and green). Green - ab177154 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab177154 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. ab177154 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154) at 1 µg/mL

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: GPHN (Gephyrin) knockout HAP1 whole cell lysate at 20 µg

    Lane 3: MCF7 whole cell lysate at 20 µg

    Lane 4: U2OS whole cell lysate at 20 µg

    Predicted band size: 80 kDa

    Observed band size: 90 kDa

  • Flow Cytometry (Intracellular) - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    Intracellular flow cytometric analysis of permeabilized 293T cells labeling Gephyrinwith ab177154 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling Gephyrin with ab177154 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing cytoplasmic staining in Jurkat cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

  • Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154), expandable thumbnail

    Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154)

    All lanes: Western blot - Anti-Gephyrin antibody [EPR12651(B)] (ab177154) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: 293T lysate at 10 µg

    Lane 3: Jurkat lysate at 10 µg

    Lane 4: SH-SY5Y lysate at 10 µg

    Lane 5: C6 lysate at 10 µg

    Lane 6: Raw264.7 lysate at 10 µg

    Lane 7: PC-12 lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 80 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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