Anti-GFAP antibody [2A5]

9 product images

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  Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

  
  Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
  
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  Western blot - Anti-GFAP antibody [2A5] (ab4648)

  Lane 1: Western blot - Anti-GFAP antibody [2A5] (ab4648)

  Lanes 2 - 8: Western blot - Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution

  Lane 2: Rat brain tissue lysate

  Lane 3: Rat spinal cord tissue lysate

  Lane 4: Mouse brain tissue lysate

  Lane 5: Mouse spinal cord tissue lysate

  Lane 6: Pig brain tissue lysate

  Lane 7: Recombinant rat GFAP protein

  Lane 8: Recombinant human GFAP protein

  Predicted band size: 49 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

  ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
  Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

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  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - Anti-MAP2 antibody ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.

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  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).

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  Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

  Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

  Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (Antigen Retrieval Buffer (100X Citrate Buffer) ab93678) for 15 minutes.
  TTo the right is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the left is white matter, both of which contain GFAP positive astrocytes. The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit.

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  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-GFAP antibody [2A5] (ab4648)

  GFAP western blot using anti-GFAP antibody [2A5] ab4648. Publication image and figure legend from Hurtado-Alvarado, G., Domínguez-Salazar, E., et al., 2016, PLoS One, PubMed 27893847.

  
  

  ab4648 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab4648 please see the product overview.

  A2A adenosine receptor antagonism attenuates GFAP overexpression induced by sleep restriction.Representative western blot images of expression of GFAP per region (cortex, hippocampus, basal nuclei, and vermis) are shown, as well as graphs depicting the relative optical density of GFAP in the following groups: control plus DMSO (Con), sleep restriction plus DMSO (SR), and sleep restriction plus SCH58261 at 0.1mg/kg (SR+SCH) (n = 3 per group). GAPDH was used for normalization. Mean ± s.e.m. Two-way ANOVA test, post hoc orthogonal contrast codes, *p<0.05 as compared to the control group; #p<0.05 with respect to the sleep restriction group.