AB190288

Anti-GFAP antibody [5C10] - Astrocyte Marker

Name: Anti-GFAP antibody [5C10] Mouse monoclonal (ab190288) | Abcam
Brand: Abcam Limited
SKU: AB190288
Price: 545 USD
Availability: InStock

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(16 Publications)

Mouse Monoclonal GFAP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Horse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

Glial fibrillary acidic protein, GFAP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

Immunohistochemical analysis of formalin-fixed adult horse brain labeling GFAP with ab190288 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

Immunofluorescent analysis of mixed neuron-glial cultures labeling GFAP with ab190288 at 1/1000 dilution. Neurofilaments are stained in green; the blue channel shows the localization of DNA.

Supplier Data

Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

All lanes:

Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (ab190288) at 1/5000 dilution

All lanes:

Rat spinal cord protein extract

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Rat, Horse, Human

Applications

ICC/IF, IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 58:e14481 PubMed40465883

2025

Low-intensity sciatic nerve-stretch injury increases nociception, anxiety-like behaviors, and astrocyte activity in male Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

G K Silva-Cardoso,P E Bello-Junior,W Lazarini-Lopes,A A Ferrarese-Tiballi,C R A Leite-Panissi

Acta neuropathologica communications 13:68 PubMed40170065

2025

PARP inhibition preserves cone photoreceptors in rd2 retina.

Applications

Unspecified application

Species

Unspecified reactive species

Pakize Nur Akkaya,María Miranda,Inmaculada Almansa,Cigdem Elmas,Dragana Trifunovic,Zohreh Hosseinzadeh,Ayse Sahaboglu

Brain pathology (Zurich, Switzerland) 35:e13327 PubMed39717017

2024

Potentiating microglial efferocytosis by MFG-E8 improves survival and neurological outcome after successful cardiopulmonary resuscitation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kunxue Zhang,Yuzhen Zhang,Zhentong Li,Jiancong Chen,Yuan Chang,Yongchuan Li,Shuxin Zeng,Sifan Pan,Suyue Pan,Kaibin Huang

IBRO neuroscience reports 17:471-479 PubMed39669223

2024

Valproic acid attenuates the severity of astrogliosis in the hippocampus of animal models of temporal lobe epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Feng,Jiamin Luo,Zhiwei Li,Yuxiao Zhao,Yamei Liu,Hongyan Zhu

Scientific reports 14:7235 PubMed38538727

2024

Obesity differentially effects the somatosensory cortex and striatum of TgF344-AD rats.

Applications

Unspecified application

Species

Unspecified reactive species

Minhal Ahmed,Aaron Y Lai,Mary E Hill,Jessica A Ribeiro,Ashley Amiraslani,JoAnne McLaurin

ACS omega 8:45288-45300 PubMed38075798

2023

Gelatin-Based Hydrogel for Three-Dimensional Neuron Culture Application.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Yun Gao,You-Ren Ji,Yi-Hua Hsu,Fang-Chun Liao,Nien-Tzu Kao,Abel Po-Hao Huang,Tai-Horng Young

Glia 71:2850-2865 PubMed37572007

2023

Increased expression of Nav1.6 of reactive astrocytes in the globus pallidus is closely associated with motor deficits in a model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Weitang Liu,Renxing Zhang,Hu Feng,Jiamin Luo,Hongyan Zhu

BMC musculoskeletal disorders 23:514 PubMed35637468

2022

Knockdown of PAR2 alleviates cancer-induced bone pain by inhibiting the activation of astrocytes and the ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Tang,Yupeng Chen,Mingzhu Yang,Qiuhui Zheng,Yaoyuan Li,Yanju Bao

Frontiers in molecular neuroscience 14:716284 PubMed34658783

2021

Proteomic Analysis Reveals Sex-Specific Protein Degradation Targets in the Amygdala During Fear Memory Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla Farrell,Madeline Musaus,Shaghayegh Navabpour,Kiley Martin,W Keith Ray,Richard F Helm,Timothy J Jarome

Glia 70:145-154 PubMed34533866

2021

Redefining varicose projection astrocytes in primates.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Falcone,Erin L McBride,William D Hopkins,Patrick R Hof,Paul R Manger,Chet C Sherwood,Stephen C Noctor,Verónica Martínez-Cerdeño

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFAP antibody [5C10] - Astrocyte Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

Product promise