Mouse Monoclonal GFAP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Horse, Rat, Human samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (AB190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119315.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (AB190288)")
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (AB190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--immunocytochemistry-immunofluorescence-img133552.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (AB190288)")
![Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--western-blot-img2674.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (AB190288)")
Publications
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- Glia 71:2850-28652023Increased expression of Nav1.6 of reactive astrocytes in the globus pallidus is closely associated with motor deficits in a model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeitang Liu,Renxing Zhang,Hu Feng,Jiamin Luo,Hongyan ZhuPubMed 37572007
- Frontiers in molecular neuroscience 14:7162842021Proteomic Analysis Reveals Sex-Specific Protein Degradation Targets in the Amygdala During Fear Memory Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKayla Farrell,Madeline Musaus,Shaghayegh Navabpour,Kiley Martin,W Keith Ray,Richard F Helm,Timothy J JaromePubMed 34658783
- Glia 70:145-1542021Redefining varicose projection astrocytes in primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Falcone,Erin L McBride,William D Hopkins,Patrick R Hof,Paul R Manger,Chet C Sherwood,Stephen C Noctor,Verónica Martínez-CerdeñoPubMed 34533866
- Nature communications 12:42202021Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongyuan Bao,Yinlong Liu,Binglin Chen,Zong Miao,Yiming Tu,Chong Li,Honglu Chao,Yangfan Ye,Xiupeng Xu,Guangchi Sun,Pengzhan Zhao,Ning Liu,Yan Liu,Xiaoming Wang,Sin Man Lam,Valerian E Kagan,Hülya Bayır,Jing JiPubMed 34244497
- Brain pathology (Zurich, Switzerland) 31:283-2962020Overexpression of the ubiquitin-editing enzyme A20 in the brain lesions of Multiple Sclerosis patients: moving from systemic to central nervous system inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Perga,Francesca Montarolo,Serena Martire,Brigitta Bonaldo,Gabriele Bono,Jessica Bertolo,Roberta Magliozzi,Antonio BertolottoPubMed 33051914
- Disease models & mechanisms 13:2020The transcription factor Nurr1 is upregulated in amyotrophic lateral sclerosis patients and SOD1-G93A mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria Valsecchi et. al.PubMed 32188741
- Muscle & nerve 60:762-7682019Assessing pathological changes within the nucleus ambiguus of horses with recurrent laryngeal neuropathy: An extreme, length-dependent axonopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra C E Draper,Stephen D Cahalan,David Goodwin,Justin Perkins,Richard J PiercyPubMed 31498901
- Journal of experimental & clinical cancer research 37:2252018LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Zhang et. al.PubMed 30208924
- Oxidative medicine and cellular longevity 2018:76421582018The Phenolic Components of improve Prognosis in Rats after Cerebral Ischemia/Reperfusion by Enhancing the Endogenous Antioxidant Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnhuan Shi et. al.PubMed 29765502
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected |
| Horse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Horse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Mouse Monoclonal GFAP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Horse, Rat, Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 5C10
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (ab190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119315.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (ab190288)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [5C10] (ab190288)
Immunohistochemical analysis of formalin-fixed adult horse brain labeling GFAP with ab190288 at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (ab190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--immunocytochemistry-immunofluorescence-img133552.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (ab190288)")
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [5C10] (ab190288)
Immunofluorescent analysis of mixed neuron-glial cultures labeling GFAP with ab190288 at 1/1000 dilution. Neurofilaments are stained in green; the blue channel shows the localization of DNA.
![Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (ab190288), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-5c10-astrocyte-marker-ab190288--western-blot-img2674.jpg "Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (ab190288)")
Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (ab190288)
GFAP Western blot staining of Rat spinal cord protein extract using mouse Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody [5C10] - Astrocyte Marker (ab190288) at 1/5000 dilution
All lanes: Rat spinal cord protein extract
Predicted band size: 49 kDa
Downloads
Product protocols
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