Skip to main content

Chicken Polyclonal GFAP antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 23 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.


Images

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogens

This product was produced with the following immunogens:

  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
  • Native Full Length Protein corresponding to Cow GFAP. Database link Q28115

Consider this alternative

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Rat
Tested

Tested
Tested

Species

Rat

Dilution info

1/2000 - 1/5000

Notes

Use 2% paraformaldehyde-fixed cells.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Chicken Polyclonal GFAP antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 23 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.

Alternative names

Key facts

Isotype

IgY

Form

Liquid

Clonality

Polyclonal

Immunogens

This product was produced with the following immunogens:

  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
  • Native Full Length Protein corresponding to Cow GFAP. Database link Q28115
Purity

IgY fraction

Concentration
Loading...
Purification notes

After repeated injections, immune eggs were collected, the IgY fractionswere purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Do not freeze this antibody unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436), expandable thumbnail
    Picture courtesy of Dr. Gerry Shaw, University of Florida

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436)

    Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab134436)

    ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com