Sheep Polyclonal GFAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Aging and disease 14:892-9032023Sexual Dimorphism in Brain Sirtuin-1 and m6A Methylated Sirtuin-1 mRNA, and in Protection with Post-Injury Anti-miR-200c treatment, after Experimental Stroke in Aged Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijun Xu,Xiaoyun Sun,Brian Griffiths,Ludmilla Voloboueva,Alex Valdes,Miles Dobrenski,Jeong-Jin Min,Creed M StaryPubMed 37191419
- Frontiers in molecular neuroscience 15:10147512022Inhibition of microRNA-200c preserves astrocyte sirtuin-1 and mitofusin-2, and protects against hippocampal neurodegeneration following global cerebral ischemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian Griffiths,Lijun Xu,Xiaoyun Sun,Majesty Greer,Isabella Murray,Creed StaryPubMed 36466801
- Science advances 7:eabk12102021An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Mu,Junying Wang,Hua He,Qifeng Li,Bing Yang,Junhui Wang,Haile Liu,Yalong Gao,Lufei Ouyang,Si Sun,Qinjuan Ren,Xinjian Shi,Wenting Hao,Qiaoman Fei,Jiang Yang,Lulin Li,Ryan Vest,Tony Wyss-Coray,Jian Luo,Xiao-Dong ZhangPubMed 34757781
- Nature communications 12:1142021Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaile Liu,Yonghui Li,Si Sun,Qi Xin,Shuhu Liu,Xiaoyu Mu,Xun Yuan,Ke Chen,Hao Wang,Kalman Varga,Wenbo Mi,Jiang Yang,Xiao-Dong ZhangPubMed 33414464
- Journal of neuropathology and experimental neurolo 75:712-7172016JC Virus Infects Neurons and Glial Cells in the Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Wüthrich et. al.PubMed 27297673
- Journal of neuropathology and experimental neurology 74:791-8032015Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Wüthrich,Stephanie Batson,Igor J KoralnikPubMed 26115192
- Journal of neuropathology and experimental neurology 71:54-652011Frequent infection of cortical neurons by JC virus in patients with progressive multifocal leukoencephalopathy.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
Constituents: Whole serum
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P14136
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Expected | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/4000 - 1/8000 | Notes - |
Species Human | Dilution info 1/4000 - 1/8000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Sheep Polyclonal GFAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
Constituents: Whole serum
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P14136
- Purity
- Whole antiserum
- Reconstitution
- Reconstitute with 50ul water and then 50ul glycerol, gently vortexing. Once resuspended, the serum will be yellow in colour.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab90601)
ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control: 40X objective.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab90601)
ab90601, at a 1/6000 dilution, staining GFAP in Rat brain tissue (left image) by Immunohistochemistry. Right image shows negative control, 40X objective.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab90601)
ab90601, at a 1/500 dilution, staining GFAP in Human brain tissue by Immunofluorescence. 40X objective.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab90601)
ab90601, at a 1/500 dilution, staining GFAP in Rat brain by Immunofluorescence. 40X objective.
Western blot - Anti-GFAP antibody (ab90601)
All lanes: Western blot - Anti-GFAP antibody (ab90601) at 1/6000 dilution
All lanes: Human brain homogenate at 5 µg
Predicted band size: 49 kDa
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