Rabbit Recombinant Monoclonal GFAP antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GFAP.
Images
![Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (AB270270), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-astrocyte-marker-ab270270--flow-cytometry-img81825.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (AB270270)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (AB270270), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-astrocyte-marker-ab270270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81307.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (AB270270)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136
Abcam Recommends
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µL for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chicken, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Rabbit Recombinant Monoclonal GFAP antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GFAP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136
- Clone number
- ASTRO/1974R
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-astrocyte-marker-ab270270--flow-cytometry-img81825.jpg "Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270)")
Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270)
GFAP Flow Cytometry staining using rabbit Anti-GFAP antibody
Flow cytometric analysis of T98G cells labeling GFAP (Blue) using ab270270 at 2 μg/106 cells, followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-astrocyte-marker-ab270270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81307.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker (ab270270)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for GFAP using ab270270 at 2 μg/ml in imunnohistochemical analysis.
Downloads
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