Rabbit Recombinant Monoclonal GFAP antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GFAP.
Images
![Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (AB270301), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-bsa-and-azide-free-ab270301--flow-cytometry-img80800.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (AB270301)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Rabbit Recombinant Monoclonal GFAP antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GFAP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136
- Carrier free
- Yes
- Clone number
- ASTRO/1974R
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab270301 is a carrier free version of Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker ab270270.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (ab270301), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-astro-1974r-bsa-and-azide-free-ab270301--flow-cytometry-img80800.jpg "Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (ab270301)")
Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (ab270301)
Flow cytometric analysis of T98G (Human brain cell line) cells labeling GFAP using Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker ab270270 at 2μg/10^6 cells followed by goat anti-rabbit IgG-CF488 (Blue); isotype control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker ab270270).
Downloads
Product protocols
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