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AB270301

Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free

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Rabbit Recombinant Monoclonal GFAP antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GFAP.

View Alternative Names

Glial fibrillary acidic protein, GFAP

1 Images
Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (AB270301)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] - BSA and Azide free (AB270301)

Flow cytometric analysis of T98G (Human brain cell line) cells labeling GFAP using ab270270 at 2μg/10^6 cells followed by goat anti-rabbit IgG-CF488 (Blue); isotype control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab270270).

  • Unconjugated

    Anti-GFAP antibody [ASTRO/1974R] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

ASTRO/1974R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GFAP.

P14136

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab270301 is a carrier free version of ab270270.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com