Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.
- Over 20 publications
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA
This product was produced with the following immunogens:
ICC/IF | |
---|---|
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/2000 - 1/5000 | Notes Use 2% paraformaldehyde-fixed cells. |
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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP, Gfap
Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.
- Over 20 publications
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA
This product was produced with the following immunogens:
After repeated injections, immune eggs were collected, the IgY fractionswere purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody
Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.
GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody
ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.
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