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Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.



- Over 20 publications


Images

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436), expandable thumbnail

Publications

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA

Form
Liquid
Clonality
Polyclonal

Immunogens

This product was produced with the following immunogens:

  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
  • Native Full Length Protein corresponding to Cow GFAP. Database link Q28115

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
1/2000 - 1/5000
Notes

Use 2% paraformaldehyde-fixed cells.

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Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Recommended products

Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.



- Over 20 publications

Key facts

Isotype
IgY
Form
Liquid
Clonality
Polyclonal
Immunogens

This product was produced with the following immunogens:

  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
  • Native Full Length Protein corresponding to Cow GFAP. Database link Q28115
Purity
IgY fraction
Concentration
Loading...
Purification notes

After repeated injections, immune eggs were collected, the IgY fractionswere purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Rat samples.

Trusted by the scientific community


Anti-GFAP (ab134436) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436), expandable thumbnail
    Picture courtesy of Dr. Gerry Shaw, University of Florida

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436)

    GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody

    Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436)

    GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody

    ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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