AB134436

Anti-GFAP antibody - Astrocyte Marker

Name: Anti-GFAP antibody - Astrocyte Marker (ab134436) | Abcam
Brand: Abcam Limited
SKU: AB134436
Price: 485 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(40 Publications)

Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody detecting GFAP in ICC/IF. Suitable for Rat.

- Over 20 publications

View Alternative Names

Glial fibrillary acidic protein, GFAP, Gfap

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436)

Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.

Picture courtesy of Dr. Gerry Shaw, University of Florida

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436)

ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Cow GFAP. Database link Q28115

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000 - 1/5000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

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Product details

What is this antibody validated in?
Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Rat samples.

Trusted by the scientific community
Anti-GFAP (ab134436) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

After repeated injections, immune eggs were collected, the IgY fractionswere purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information Gfap

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7994 PubMed40866328

2025

Microglial replacement in a Sandhoff disease mouse model reveals myeloid-derived β-hexosaminidase is necessary for neuronal health.

Applications

Unspecified application

Species

Unspecified reactive species

Kate I Tsourmas,Claire A Butler,Nellie E Kwang,Zachary R Sloane,Koby J G Dykman,Ghassan O Maloof,Biswa P Choudhury,Mousumi Paulchakrabarti,Christiana A Prekopa,Emily Z Tabaie,Robert P Krattli,Sanad M El-Khatib,Vivek Swarup,Munjal M Acharya,Lindsay A Hohsfield,Kim N Green

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70331 PubMed40731189

2025

Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoduo Zhao,Jiayi Yu,Bin Xu,Zhi Xu,Xia Lei,Shilong Han,Shangfei Luo,Can Zhang,Guoping Peng,Jing Li,Jie Yu,Yi Ling,Zhongqin Fan,Wei Mo,Ying Yang,Jing Zhang

Molecular neurobiology 62:15943-15968 PubMed40728771

2025

Necl-4/CADM4 regulates GABAergic synaptic strength on GABAergic inhibitory neurons via ErbB4 activation and prevents neuronal impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Ryouhei Komaki,Hajime Shiotani,Toshihiko Kuriu,Takeshi Kameyama,Muneaki Miyata,Shin Kedashiro,Kimitaka Katanazaka,Shota Nishii,Norio Chihara,Riki Matsumoto,Michinori Koebis,Atsu Aiba,Kiyohito Mizutani,Yoshimi Takai

Frontiers in pharmacology 16:1510250 PubMed40584618

2025

The anti-depressive role of the Pei Yuan Kai Yu formula in cerebral small vessel disease based on gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Yang,Yutian Ao,Yannan Li,Baoan Dai,Junnan Li,Wenzhe Duan,Kaiqiang Dong,Zhenyun Han,Rongjuan Guo

Materials today. Bio 32:101895 PubMed40502366

2025

Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshui Mao,Liangliang Chen,Genying Zhuang,Zhiqiang Jin,Shoukun Wu,Mingshuai Ying,Lingchao Sheng,Jianwei Lv,Qiuchen Bi,Jingjia Ye

Molecular neurodegeneration 20:9 PubMed39844286

2025

APOE Christchurch enhances a disease-associated microglial response to plaque but suppresses response to tau pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Kristine M Tran,Nellie E Kwang,Claire A Butler,Angela Gomez-Arboledas,Shimako Kawauchi,Cassandra Mar,Donna Chao,Rocio A Barahona,Celia Da Cunha,Kate I Tsourmas,Zechuan Shi,Shuling Wang,Sherilyn Collins,Amber Walker,Kai-Xuan Shi,Joshua A Alcantara,Jonathan Neumann,Duc M Duong,Nicholas T Seyfried,Andrea J Tenner,Frank M LaFerla,Lindsay A Hohsfield,Vivek Swarup,Grant R MacGregor,Kim N Green

Nature communications 15:10865 PubMed39738113

2024

Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Ding,Yu Wu,Anna Vainshtein,Victoria Rodriguez,Emily Ricco,James T Okoh,Yanhong Liu,Daniel C Kraushaar,Elior Peles,Matthew N Rasband

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:85-101 PubMed39068534

2024

Critical role of Slc22a8 in maintaining blood-brain barrier integrity after experimental cerebral ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Liu,Xiang Li,Chang Cao,Haojie Ding,Xuan Shi,Juyi Zhang,Haiying Li

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:4914-4934 PubMed38506634

2024

The Abca7 variant reduces Aβ generation, plaque load, and neuronal damage.

Applications

Unspecified application

Species

Unspecified reactive species

Claire A Butler,Adrian Mendoza Arvilla,Giedre Milinkeviciute,Celia Da Cunha,Shimako Kawauchi,Narges Rezaie,Heidi Y Liang,Dominic Javonillo,Annie Thach,Shuling Wang,Sherilyn Collins,Amber Walker,Kai-Xuan Shi,Jonathan Neumann,Angela Gomez-Arboledas,Caden M Henningfield,Lindsay A Hohsfield,Mark Mapstone,Andrea J Tenner,Frank M LaFerla,Ali Mortazavi,Grant R MacGregor,Kim N Green

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:2794-2816 PubMed38426371

2024

Genetic diversity promotes resilience in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Neelakshi Soni,Lindsay A Hohsfield,Kristine M Tran,Shimako Kawauchi,Amber Walker,Dominic Javonillo,Jimmy Phan,Dina Matheos,Celia Da Cunha,Asli Uyar,Giedre Milinkeviciute,Angela Gomez-Arboledas,Katelynn Tran,Catherine C Kaczorowski,Marcelo A Wood,Andrea J Tenner,Frank M LaFerla,Gregory W Carter,Ali Mortazavi,Vivek Swarup,Grant R MacGregor,Kim N Green

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFAP antibody - Astrocyte Marker

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436)

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB134436)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (40)

Product promise