Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.
- Over 20 publications
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- iScience 26:1080102023Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi TakaiPubMed 37829206
- Cells 12:2023Interactions between the Astrocytic Volume-Regulated Anion Channel and Aquaporin 4 in Hyposmotic Regulation of Vasopressin Neuronal Activity in the Supraoptic Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu,Xiao-Ran Wang,Yun-Hao Jiang,Tong Li,Shuo Ling,Hong-Yang Wang,Jia-Wei Yu,Shu-Wei Jia,Xiao-Yu Liu,Chun-Mei Hou,Vladimir Parpura,Yu-Feng WangPubMed 37443757
- Advanced biology 7:e23000982023Single-Cell Sequencing Reveals the Optimal Time Window for Anti-Inflammatory Treatment in Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjia Ye,Zhengfa Wen,Tianxin Wu,Liangliang Chen,Lingchao Sheng,Chenhuan Wang,Chong Teng,Bingbing Wu,Jian Xu,Wei WeiPubMed 37085744
- Bioengineering & translational medicine 8:e104482023Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Jin,Yayu Wu,Haipeng Zhang,Bai Xu,Wenbin Liu,Chunnan Ji,Panpan Li,Zhenni Chen,Bing Chen,Jiayin Li,Xianming Wu,Peipei Jiang,Yali Hu,Zhifeng Xiao,Yannan Zhao,Jianwu DaiPubMed 36925694
- Molecular neurodegeneration 18:122023A Trem2 mouse model without cryptic splicing drives age- and disease-dependent tissue damage and synaptic loss in response to plaques.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristine M Tran,Shimako Kawauchi,Enikö A Kramár,Narges Rezaie,Heidi Yahan Liang,Jasmine S Sakr,Angela Gomez-Arboledas,Miguel A Arreola,Celia da Cunha,Jimmy Phan,Shuling Wang,Sherilyn Collins,Amber Walker,Kai-Xuan Shi,Jonathan Neumann,Ghassan Filimban,Zechuan Shi,Giedre Milinkeviciute,Dominic I Javonillo,Katelynn Tran,Magdalena Gantuz,Stefania Forner,Vivek Swarup,Andrea J Tenner,Frank M LaFerla,Marcelo A Wood,Ali Mortazavi,Grant R MacGregor,Kim N GreenPubMed 36803190
- The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5641-56542022Unbalanced Regulation of Sec22b and Ykt6 Blocks Autophagosome Axonal Retrograde Flux in Neuronal Ischemia-Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiying Li,Xiang Li,Zhongmou Xu,Jinxin Lu,Chang Cao,Wanchun You,Zhengquan Yu,Haitao Shen,Gang ChenPubMed 35654605
- Frontiers in neuroscience 15:7852762022Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominic I Javonillo,Kristine M Tran,Jimmy Phan,Edna Hingco,Enikö A Kramár,Celia da Cunha,Stefania Forner,Shimako Kawauchi,Giedre Milinkeviciute,Angela Gomez-Arboledas,Jonathan Neumann,Crystal E Banh,Michelle Huynh,Dina P Matheos,Narges Rezaie,Joshua A Alcantara,Ali Mortazavi,Marcelo A Wood,Andrea J Tenner,Grant R MacGregor,Kim N Green,Frank M LaFerlaPubMed 35140584
- Glia 70:337-3532021Astrocytic VEGFA: An essential mediator in blood-brain-barrier disruption in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoyu Lan,Pan Wang,Robin Barry Chan,Zongran Liu,Zhenwei Yu,Xiaodan Liu,Ying Yang,Jing ZhangPubMed 34713920
- Scientific data 8:2702021Systematic phenotyping and characterization of the 5xFAD mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefania Forner,Shimako Kawauchi,Gabriela Balderrama-Gutierrez,Enikö A Kramár,Dina P Matheos,Jimmy Phan,Dominic I Javonillo,Kristine M Tran,Edna Hingco,Celia da Cunha,Narges Rezaie,Joshua A Alcantara,David Baglietto-Vargas,Camden Jansen,Jonathan Neumann,Marcelo A Wood,Grant R MacGregor,Ali Mortazavi,Andrea J Tenner,Frank M LaFerla,Kim N GreenPubMed 34654824
- Neuroscience bulletin 38:290-3022021Polysaccharide Krestin Prevents Alzheimer's Disease-type Pathology and Cognitive Deficits by Enhancing Monocyte Amyloid-β Processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi-Han Chen,Chen-Yang He,Ying-Ying Shen,Gui-Hua Zeng,Ding-Yuan Tian,Yuan Cheng,Man-Yu Xu,Dong-Yu Fan,Cheng-Rong Tan,An-Yu Shi,Xian-Le Bu,Yan-Jiang WangPubMed 34611829
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogens
This product was produced with the following immunogens:
Consider this alternative
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Rat | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 - 1/5000 | Notes Use 2% paraformaldehyde-fixed cells. |
Associated Products
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Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP, Gfap
Recommended products
Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody that is used to detect GFAP in ICC/IF. Suitable for Rat samples.
- Over 20 publications
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
This product was produced with the following immunogens:
- Purity
- IgY fraction
- Concentration
- Purification notes
After repeated injections, immune eggs were collected, the IgY fractionswere purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GFAP antibody (ab134436) is a chicken polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Rat samples.
Trusted by the scientific community
Anti-GFAP (ab134436) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Picture courtesy of Dr. Gerry Shaw, University of FloridaImmunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436)
GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody
Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab134436)
GFAP Immunocytochemistry/ Immunofluorescence staining using chicken Anti-GFAP antibody
ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.
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