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Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (AB254082), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (AB254082), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (AB254082), expandable thumbnail
  • Western blot - Anti-GFAP antibody - Astrocyte Marker (AB254082), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer
Standard buffer
Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Predicted
Predicted
Mouse
Expected
Tested
Rat
Tested
Tested
Cow
Predicted
Predicted

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Cow, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Recommended products

Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
Purity
Whole antiserum
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)

    Immunofluorescence of rat cerebellum labeling GFAP with ab254082 (green) at a 1/5000 dilution. Co-labeled with anti-MeCP2 (1/500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)

    Immunolabeling of mouse cortical cultures labeling GFAP with ab254082 (green) at a 1/1000 dilution and anti-MAP2 (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)

    Immunofluorescence of rat cerebellum tissue labeling GFAP with ab254082 (green) at a 1/1000 dilution. Co-labeled with anti- calbindin (red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.

  • Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082), expandable thumbnail

    Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082)

    GFAP Western blot staining of Rat cortical lysate using rabbit Anti-GFAP antibody

    All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082) at 1/1000 dilution

    All lanes: Rat cortical lysate

    Predicted band size: 49 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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