Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.
WB | ICC/IF | |
---|---|---|
Human | Predicted | Predicted |
Mouse | Expected | Tested |
Rat | Tested | Tested |
Cow | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Human | Dilution info - | Notes - |
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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP, Gfap
Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunofluorescence of rat cerebellum labeling GFAP with ab254082 (green) at a 1/5000 dilution. Co-labeled with anti-MeCP2 (1/500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunolabeling of mouse cortical cultures labeling GFAP with ab254082 (green) at a 1/1000 dilution and anti-MAP2 (red).
Immunofluorescence of rat cerebellum tissue labeling GFAP with ab254082 (green) at a 1/1000 dilution. Co-labeled with anti- calbindin (red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
GFAP Western blot staining of Rat cortical lysate using rabbit Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082) at 1/1000 dilution
All lanes: Rat cortical lysate
Predicted band size: 49 kDa
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