Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.
Images
Publications
Filter by
- Frontiers in immunology 11:5786482020Human Commensal Ameliorates Disease as Effectively as Interferon-Beta in the Experimental Autoimmune Encephalomyelitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShailesh K Shahi et. al.PubMed 33362764
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Expected | Tested |
| Rat | Tested | Tested |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP, Gfap
Recommended products
Rabbit Polyclonal GFAP antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow GFAP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Cow GFAP. Database link Q28115
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)
Immunofluorescence of rat cerebellum labeling GFAP with ab254082 (green) at a 1/5000 dilution. Co-labeled with anti-MeCP2 (1/500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)
Immunolabeling of mouse cortical cultures labeling GFAP with ab254082 (green) at a 1/1000 dilution and anti-MAP2 (red).
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab254082)
Immunofluorescence of rat cerebellum tissue labeling GFAP with ab254082 (green) at a 1/1000 dilution. Co-labeled with anti- calbindin (red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082)
GFAP Western blot staining of Rat cortical lysate using rabbit Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab254082) at 1/1000 dilution
All lanes: Rat cortical lysate
Predicted band size: 49 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com