AB53554

Anti-GFAP antibody - Astrocyte Marker

Name: Anti-GFAP (Glial Fibrillary Acidic Protein) antibody (ab53554) | Abcam
Brand: Abcam Limited
SKU: AB53554
Price: 450 USD
Availability: InStock
Rating: 5 (24 reviews)

(24 Reviews)

(543 Publications)

Anti-GFAP antibody (ab53554) is a goat polyclonal antibody detecting GFAP in Western Blot. Suitable for Human, Mouse, Rat.

- Over 390 publications
- Trusted since 2007

View Alternative Names

Glial fibrillary acidic protein, GFAP

2 Images

Supplier Data

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

Lane 1 : 0.001 µg/mL Lane 2 : 0.1 µg/mL Lane 1 : 0.03 µg/mL Lysate in RIPA buffer. Detected by chemiluminescence

All lanes:

Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554)

Lane 1:

Human Cerebellum tissue lysate at 35 µg

Lane 2:

Mouse brain tissue lysate at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

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AbReview47180****

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

All lanes:

Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554) at 1/1000 dilution

All lanes:

Human Tissue lysate - other (Frontal Cortex) at 30 µg

Secondary

All lanes:

Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye

Predicted band size: 49 kDa

Observed band size: 40-50 kDa

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This image was courtesy of an annonymous Abreview

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human GFAP aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P14136

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature." }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Find all reagents to label astrocytes in our "Astrocytes markers guide".

Anti-GFAP antibody (ab53554) is a Goat Polyclonal antibody and is validated for use in WB in cow, human, mouse, rat samples.

Anti-GFAP antibody (ab53554) has been cited over 392 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-GFAP antibody (ab53554) has high sensitivity and specificity.

Anti-GFAP antibody (ab53554) has 24 independent reviews from customers.

Anti-GFAP antibody (ab53554) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in 100 ug selling sizes.

GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI), and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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Publications (543)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:203 PubMed41024170

2025

Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Azzurra Cottarelli,Danny Jamoul,Mary Claire Tuohy,Sanjid Shahriar,Michael Glendinning,Grace Prochilo,Aimee L Edinger,Ahmet Arac,Dritan Agalliu

Acta neuropathologica communications 13:205 PubMed41024271

2025

Blocking axon-glial mechanotransduction to prevent concussive brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Sun,Di Ma,Jacob Hansen,Jeffrey R Tonniges,Hongzhen Hu,Liwen Zhang,Chen Gu

Signal transduction and targeted therapy 10:295 PubMed40931022

2025

Astrocytic monoamine oxidase B (MAOB)-gamma-aminobutyric acid (GABA) axis as a molecular brake on repair following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Yeong Lee,Jung Moo Lee,Hye-Lan Lee,Jiyeon Park,Heeyoung An,Eun Kyung Park,Sae Yeon Hwang,Sol Lip Yoon,Gwang Yong Hwang,Keung Nyun Kim,Min-Ho Nam,Seung Eun Lee,Hyunji Kang,Joungha Won,Bo Ko Jang,Elijah Hwejin Lee,SunYeong Choi,Mingu Gordon Park,Sang Wook Kim,Ki Duk Park,SeungHwan Lee,C Justin Lee,Yoon Ha

Neurotrauma reports 6:586-599 PubMed40933834

2025

Applications

Unspecified application

Species

Unspecified reactive species

Aria W Tarudji,Brandon Z McDonald,Evan Curtis,Connor Gee,Forrest M Kievit

ACS sensors 10:6426-6435 PubMed40838718

2025

Inkjet-Printed 3D Sensor Arrays with FIB-Induced Electrode Refinement for Low-Noise Amperometric Recordings in hiPSC-Derived Brain Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Inola Kopic,Hu Peng,Sebastian Schmidt,Oleksandr Berezin,Senyao Wang,Gil G Westmeyer,Bernhard Wolfrum

Nature 646:452-461 PubMed40836081

2025

Axonal injury is a targetable driver of glioblastoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Clements,Wenhao Tang,Zan Florjanic Baronik,Holly Simpson Ragdale,Roger Oria,Dimitrios Volteras,Ian J White,Gordon Beattie,Imran Uddin,Tchern Lenn,Rachel Lindsay,Sara Castro Devesa,Saketh R Karamched,Mark F Lythgoe,Vahid Shahrezaei,Valerie M Weaver,Ryoichi Sugisawa,Federico Roncaroli,Samuel Marguerat,Ciaran S Hill,Simona Parrinello

Nature communications 16:7551 PubMed40813385

2025

Astrocyte priming enhances microglial Aβ clearance and is compromised by APOE4.

Applications

Unspecified application

Species

Unspecified reactive species

Se-In Lee,Jichang Yu,Hyein Lee,Buyun Kim,Min Jun Jang,Hyeonbin Jo,Na Yeon Kim,Malk Eun Pak,Jae Kwang Kim,Sukhee Cho,Hong-Hee Won,Min Soo Kim,Fan Gao,Younghoon Go,Jinsoo Seo

Translational neurodegeneration 14:41 PubMed40814010

2025

Targeted delivery of BACE1 siRNA for synergistic treatment of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohan Li,Jun Yang,Jianan Li,Shuxuan Zhao,Shaoping Jiang,Weimin Liu,Xinjian Li,Simeng Zhang,Haiyan Du,Junjun Ni,Yuanyu Huang,Hong Qing,Shaobo Ruan

International journal of molecular sciences 26: PubMed40650297

2025

bFGF-Mediated Inhibition of Astrocytes' Optogenetic Activation Impairs Neuronal Repair in Female Rats After Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xinfa Shao,Yangqianbo Yao,Victoria Shi,Qian Suo,Shengju Wu,Han Wang,Muyassar Mamtilahun,Wanlu Li,Yaohui Tang,Guo-Yuan Yang,Qun Xu,Zhijun Zhang

Nature communications 16:5621 PubMed40595567

2025

Connexin43 hemichannel blockade turns microglia neuroprotective and mitigates cognitive deficits in a mouse model of amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Hui Li,Wenjie Zhang,Shi Tao,Qi Wang,Xuan Zhang,Mi Zhou,Xiaomin Huang,Chenmeng Wang,Yong Tang,Hui Chen,Alexei Verkhratsky,Zhengbao Zha,Jianqin Niu,Chenju Yi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFAP antibody - Astrocyte Marker

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB53554)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (543)

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