Anti-GFAP antibody is a goat polyclonal antibody that is used to detect GFAP in Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 390 publications
- Tried and trusted by researchers since 2007
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- Aging and disease 15:1384-13972024Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu Chen,Qi Xie,Lexing Xie,Jiacheng Huang,Linlin Hu,Hui Lu,Peixia Shi,Qian He,Qin Zhang,Changxiong Gong,Shuang Zhang,Bingqiao Wang,Guoqiang Yang,Qingwu YangPubMed 37196136
- Scientific reports 13:205412023Long-term HIF-1α stabilization reduces respiration, promotes mitophagy, and results in retinal cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNana Yaa Nsiah,Autumn B Morgan,Nina Donkor,Denise M InmanPubMed 37996657
- Communications biology 6:11512023A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomas Lagunas,Stephen P Plassmeyer,Anthony D Fischer,Ryan Z Friedman,Michael A Rieger,Din Selmanovic,Simona Sarafinovska,Yvette K Sol,Michael J Kasper,Stuart B Fass,Alessandra F Aguilar Lucero,Joon-Yong An,Stephan J Sanders,Barak A Cohen,Joseph D DoughertyPubMed 37953348
- Glia 72:300-3212023Impaired cortical neuronal homeostasis and cognition after diffuse traumatic brain injury are dependent on microglia and type I interferon responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan M Packer,Chelsea E Bray,Nicolas B Beckman,Lynde M Wangler,Amara C Davis,Ethan J Goodman,Nathaniel E Klingele,Jonathan P GodboutPubMed 37937831
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 54:965-9712023[Photobiomodulation Promotes Hippocampal Neurogenesis and Improves Cognitive Function and Anti-Inflammatory Injury in Rats With Chronic Cerebral Hypoperfusion].Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyi Tu,Yuanyuan Huang,Ying Huang,Meng Wu,Ruimin WangPubMed 37866954
- Journal of neuroinflammation 20:2222023Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning ZhangPubMed 37777772
- Cellular and molecular life sciences : CMLS 80:2892023Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunjian Dai,Taofeng Wei,Yuwen Huang,Yun Bei,Haoran Lin,Zexu Shen,Lingyan Yu,Mingdong Yang,Huimin Xu,Wei He,Zheng Lin,Haibin DaiPubMed 37690046
- Stem cell research & therapy 14:2322023Transplanted ENSCs form functional connections with intestinal smooth muscle and restore colonic motility in nNOS-deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyo Hotta,Ahmed Rahman,Sukhada Bhave,Rhian Stavely,Weikang Pan,Shriya Srinivasan,Geoffrey de Couto,Luis Rodriguez-Borlado,Richard Myers,Alan J Burns,Allan M GoldsteinPubMed 37667277
- Cell death & disease 14:5542023Autophagy deficiency protects against ocular hypertension and neurodegeneration in experimental and spontanous glaucoma mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela Dixon,Myoung Sup Shim,April Nettesheim,Aislyn Coyne,Chien-Chia Su,Haiyan Gong,Paloma B LitonPubMed 37620383
- Frontiers in neuroscience 17:12446792023Retinal astrocyte morphology predicts integration of vascular and neuronal architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph M Holden,Lauren K Wareham,David J CalkinsPubMed 37621717
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GFAP aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14136
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.001-0.1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
Species Rat | Dilution info 0.001-0.1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
Species Human | Dilution info 0.001-0.1 µg/mL | Notes A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Anti-GFAP antibody is a goat polyclonal antibody that is used to detect GFAP in Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 390 publications
- Tried and trusted by researchers since 2007
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GFAP aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14136
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Find all reagents to label astrocytes in our "Astrocytes markers guide".
Anti-GFAP antibody (ab53554) is a Goat Polyclonal antibody and is validated for use in WB in cow, human, mouse, rat samples.
Anti-GFAP antibody (ab53554) has been cited over 392 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-GFAP antibody (ab53554) has high sensitivity and specificity.
Anti-GFAP antibody (ab53554) has 24 independent reviews from customers.
Anti-GFAP antibody (ab53554) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in 100 ug selling sizes.
GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI), and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554)
GFAP Western blot staining using goat Anti-GFAP antibody
Lane 1: 0.001 µg/mL
Lane 2: 0.1 µg/mL
Lane 1: 0.03 µg/mL
Lysate in RIPA buffer. Detected by chemiluminescenceAll lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554)
Lane 1: Human Cerebellum tissue lysate at 35 µg
Lane 2: Mouse brain tissue lysate at 35 µg
Lane 3: Rat brain tissue lysate at 35 µg
This image was courtesy of an annonymous customer reviewWestern blot - Anti-GFAP antibody - Astrocyte Marker (ab53554)
GFAP Western blot staining of Human Tissue lysate - other (Frontal Cortex) using goat Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab53554) at 1/1000 dilution
All lanes: Human Tissue lysate - other (Frontal Cortex) at 30 µg
SecondaryAll lanes: Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 49 kDa
Observed band size: 40-50 kDa
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