Name: Anti-GFAP antibody - Astrocyte Marker
SKU: ab7260
Rating: 5 (103 reviews)

Anti-GFAP antibody is a rabbit polyclonal antibody that is used to detect GFAP in ICC/IF, IHC (PFA fixed), IHC-P, IP, Western blot. Suitable for Mouse, Rat samples.

- Tried and trusted by researchers since 2002

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB7260), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.03% Sodium azide
Form: Liquid
Clonality: Polyclonal

Immunogen

Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	IHC (PFA fixed)
Human	Predicted	Predicted	Predicted	Predicted	Predicted
Mouse	Tested	Tested	Tested	Expected	Tested
Rat	Tested	Tested	Expected	Tested	Expected
Cow	Predicted	Predicted	Predicted	Predicted	Predicted
Horse	Predicted	Predicted	Predicted	Predicted	Predicted
Mammals	Predicted	Predicted	Predicted	Predicted	Predicted
Pig	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000 - 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Horse, Cow, Human, Pig, Mammals	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Horse, Cow, Human, Pig, Mammals	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Horse, Cow, Human, Pig, Mammals	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/10000	Notes This lower 48kDa band is thought to be a degradation product.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Horse, Cow, Human, Pig, Mammals	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Horse, Cow, Human, Pig, Mammals	Dilution info -	Notes -

Associated Products

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Target data

See full target information GFAP

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.03% Sodium azide
Form: Liquid
Clonality: Polyclonal
Immunogen: Recombinant Full Length Protein corresponding to Human GFAP. Database link P14136
Purity: Whole antiserum
Specificity: Specifically recognizes mammalian GFAP on western blots and immunocytochemically. Detects a band of 55kDa corresponding to GFAP and also a GFAP derived 48kDa band. Some customers have successfully used ab7260 on Zebrafish lysates; however we have conflicting data to suggest that not all batches will be suitable for work on Zebrafish. For further information, please contact Abcam Scientific Support.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Find all reagents to label astrocytes in our "Astrocytes markers guide".

Anti-GFAP antibody (ab7260) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC (PFA fixed), IHC-P, IP and WB.

Anti-GFAP antibody (ab7260) was first used in a scientific publication in 2006 and has been cited over 1203 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-GFAP antibody (ab7260) has high sensitivity and specificity.

Anti-GFAP antibody (ab7260) has 103 independent reviews from customers.

Anti-GFAP antibody (ab7260) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in 50 µL selling sizes.

GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI) and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
IHC image of GFAP staining in a formalin fixed, paraffin embedded normal mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7260 at 1/2000 dilution for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of an anonymous customer review
Western blot - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Western blot staining using rabbit Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab7260) at 1/5000 dilution
Lanes 1 - 3: Rat thoracotomy, spinal cord homogenate at 30 µg
Lanes 4 - 5: Rat thoracotomy sham, spinal cord homogenate at 30 µg
Lanes 6 - 7: Rat nerve transect sham, spinal cord homogenate at 30 µg
Lanes 8 - 9: Rat nerve transect, spinal cord homogenate at 30 µg
Secondary
All lanes: HRP conjugated goat anti-rabbit at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 53 kDa
Exposure time: 1min
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody
ab7260 at 1/10000 dilution staining mouse cortical astrocytes by Immunocytochemistry.
The cells were permeabilized with Triton/HEPES buffer prior to primary application. The antibody was incubated with the cells for 18 hours and then bound antibody was detected with an Alexa Fluor ^® 488 conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submited by Charmaine Noonan.
This image is courtesy of a customer review submitted by Ms Nancy Nutile-McMenemy
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody
ab7260 staining rat pup cortical preps by ICC/IF.
The preps were grown for 14 days in culture and plated onto coverslips. The preps were acid/alcohol fixed and blocked prior to incubation with ab7260. Bound antibody was detected using an Alexa Fluor ^®488 conjugated goat polyclonal antibody. Nuclei were visualized using DAPI.
Immunoprecipitation - Anti-GFAP antibody - Astrocyte Marker (ab7260)
Immunoprecipitation side-by-side comparison with the recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
This immunoprecipitation image is a comparison between ab7260 and the alternative recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Left side - Recombinant multiclonal to GFAP - Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
GFAP was immunoprecipitated from 0.35 mg mouse brain lysate with Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10 μg.
Lane 2: Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Right side - Polyclonal antibody to [GFAP] - ab7260
Same testing conditions as Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-free
All lanes: Immunoprecipitation - Anti-GFAP antibody - Astrocyte Marker (ab7260)
Predicted band size: 49 kDa
This image was kindly supplied by Dr Vladimir Milenkovic by customer review
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
ab7260 staining GFAP in mouse eye tissue sections by Immunohistochemistry (paraffin embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C; followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor^® 488 used at a 1/5000 dilution.
The retinal layers are: ganglion cells layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), and photoreceptor outer segments (ROS). Nuclei were counterstained with DAPI.
Western blot - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Western blot staining using rabbit Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody - Astrocyte Marker (ab7260) at 1/5000 dilution
Lane 1: Rat brain lysate
Lane 2: Rat spinal cord lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse spinal cord lysate
Predicted band size: 49 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody
ICC/IF side-by-side comparison with the recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
This ICC/IF image is a comparison between ab7260 and the alternative recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Left side - Recombinant multiclonal to GFAP - Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling GFAP with Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at 1/500 (0.938 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary astrocytes. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
ab10062 Anti-GFAP mouse monoclonal antibody at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red) to counterstain. The nuclear counterstain was DAPI (Blue).
Negative control 1: Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at a 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/500 dilution.
Negative control 2: ab10062 at a 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at a 1/1000 dilution.
Right side - Polyclonal antibody to [GFAP] - ab7260
Same testing conditions as Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-free
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
IHC-P side-by-side comparison with the recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
This IHC-P image is a comparison between ab7260 and the alternative recombinant multiclonal antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Left side - Recombinant multiclonal to GFAP - Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054
IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat brain tissue section, performed on a Leica Bond™ system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at 1/2000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Right side - Polyclonal antibody to [GFAP] - ab7260
Same testing conditions as Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-free
Yoon, H. et al PLoS One. 2017 Jul 10;12(7):e0180697. doi: 10.1371/journal.pone.0180697. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
Increases in GFAP after demyelinating injury are greater in the spinal cord compared to brain
Photomicrographs show immunoreactivity for GFAP or ALDH1L1 in (A) the corpus callosum, or (B) the dorsal column white matter of adult mice at base line, and at 14 d after microinjection of the demyelinating agent lysolecithin. Histograms show the percent area of GFAP immunofluorescence, and expression of GFAP/ALDH1L1+ astrocyte, was significantly greater in the spinal cord compared to the corpus callosum 14d post-lysolecithin lesion.
GFAP was detected using ab7260.
(From Figure 4A and 4B of Hoon et al)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat brain tissue section, performed on a Leica Bond™ system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7260 at 1/2000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of a customer review submitted by Chia-Li Lin.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody
ab7260 staining GFAP in cells from mouse brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 in PBS and blocked with 1% BSA for 40 minutes at 25°C. Samples were incubated with primary antibody (1/1200 in TBS) for 24 hours at 4°C. Goat Anti-Rabbit IgG H&L (DyLight^® 488) (Goat Anti-Rabbit IgG H&L (DyLight® 488) ab96883) was used as the secondary antibody at a dilution of 1/200.
This image is courtesy of a customer review submitted by Mr Osama Mohsen
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GFAP antibody
ab7260 staining rat brain tissue sections by IHC-P.
Sections were fixed in formaldehyde and blocked with a commercialy available blocking agent prior to incubating with ab7260, diluted 1/5000 for 20 hours at 4°C. An HRP conjugated mouse polyclonal (universal HRP polymer detection) antibody was used as the secondary.
Immunohistochemistry (PFA fixed) - Anti-GFAP antibody - Astrocyte Marker (ab7260)
GFAP Immunohistochemistry (PFA fixed) staining using rabbit Anti-GFAP antibody
GFAP antibody ab7260 was used with Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: GFAP.
Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.
To use this antibody with tissue clearing, use Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298. For 1 mm brain sections, we recommend a starting dilution of 1:1000, and also using Goat Anti-Rabbit IgG H&L Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1:400.