AB90601

Anti-GFAP antibody - Astrocyte Marker

Name: Anti-GFAP antibody - Astrocyte Marker (ab90601) | Abcam
Brand: Abcam Limited
SKU: AB90601
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(7 Publications)

Sheep Polyclonal GFAP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Glial fibrillary acidic protein, GFAP

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB90601)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB90601)

ab90601, at a 1/500 dilution, staining GFAP in Human brain tissue by Immunofluorescence. 40X objective.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB90601)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB90601)

ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control : 40X objective.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB90601)

ab90601, at a 1/6000 dilution, staining GFAP in Rat brain tissue (left image) by Immunohistochemistry. Right image shows negative control, 40X objective.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB90601)

ab90601, at a 1/500 dilution, staining GFAP in Rat brain by Immunofluorescence. 40X objective.

Unknown

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB90601)

All lanes:

Western blot - Anti-GFAP antibody - Astrocyte Marker (ab90601) at 1/6000 dilution

All lanes:

Human brain homogenate at 5 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GFAP aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P14136

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000 - 1/8000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000 - 1/8000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with glycerol at 50µL

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease 14:892-903 PubMed37191419

2023

Sexual Dimorphism in Brain Sirtuin-1 and m6A Methylated Sirtuin-1 mRNA, and in Protection with Post-Injury Anti-miR-200c treatment, after Experimental Stroke in Aged Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Xu,Xiaoyun Sun,Brian Griffiths,Ludmilla Voloboueva,Alex Valdes,Miles Dobrenski,Jeong-Jin Min,Creed M Stary

Frontiers in molecular neuroscience 15:1014751 PubMed36466801

2022

Inhibition of microRNA-200c preserves astrocyte sirtuin-1 and mitofusin-2, and protects against hippocampal neurodegeneration following global cerebral ischemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Griffiths,Lijun Xu,Xiaoyun Sun,Majesty Greer,Isabella Murray,Creed Stary

Science advances 7:eabk1210 PubMed34757781

2021

An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Mu,Junying Wang,Hua He,Qifeng Li,Bing Yang,Junhui Wang,Haile Liu,Yalong Gao,Lufei Ouyang,Si Sun,Qinjuan Ren,Xinjian Shi,Wenting Hao,Qiaoman Fei,Jiang Yang,Lulin Li,Ryan Vest,Tony Wyss-Coray,Jian Luo,Xiao-Dong Zhang

Nature communications 12:114 PubMed33414464

2021

Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions.

Applications

Unspecified application

Species

Unspecified reactive species

Haile Liu,Yonghui Li,Si Sun,Qi Xin,Shuhu Liu,Xiaoyu Mu,Xun Yuan,Ke Chen,Hao Wang,Kalman Varga,Wenbo Mi,Jiang Yang,Xiao-Dong Zhang

Journal of neuropathology and experimental neurolo 75:712-717 PubMed27297673

2016

JC Virus Infects Neurons and Glial Cells in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Wüthrich,Stephanie Batson,Matthew P Anderson,Lon R White,Igor J Koralnik

Journal of neuropathology and experimental neurology 74:791-803 PubMed26115192

2015

Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Wüthrich,Stephanie Batson,Igor J Koralnik

Journal of neuropathology and experimental neurology 71:54-65 PubMed22157619

2011

Frequent infection of cortical neurons by JC virus in patients with progressive multifocal leukoencephalopathy.

Applications

IHC-P

Species

Human

Christian Wüthrich,Igor J Koralnik

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFAP antibody - Astrocyte Marker

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB90601)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB90601)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody - Astrocyte Marker (AB90601)

Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody - Astrocyte Marker (AB90601)

Western blot - Anti-GFAP antibody - Astrocyte Marker (AB90601)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Reconstitution

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise