Name: Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric)
SKU: ab302644
Rating: 5 (1 reviews)

Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644) is a goat monoclonal antibody detecting GFAP in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for House Mouse, Human, Norway Rat.

- Biophysical QC for unrivalled batch-batch consistency

Images

Western blot - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (AB302644), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Goat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ICC/IF	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Expected	Tested	Tested	Expected	Expected
Mouse	Not recommended	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Tested	Tested	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/250	Notes -
Species Mouse	Dilution info 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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3 products for Alternative Version

Target data

See full target information GFAP

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP

Key facts

Isotype: IgG
Host species: Goat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR1034Y-GtIgG
Purification technique: Ion exchange chromatography
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?
Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644) is a goat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in House Mouse, Human, Norway Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR1034Y-GtIgG] also available for your convenience: ab302644, Carrier free - ab302645

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: Lane 1: 3.25 seconds
Lane 2-3: 70 seconds
All lanes: Western blot - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Rat cerebellum tissue lysate at 20 µg
Secondary
All lanes: Rabbit anti-Goat IgG (H+L), Peroxidase conjugated at 1/5000 dilution
Observed band size: 50 kDa
Exposure time: 70s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) Donkey Anti-Goat IgG H&L (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on mouse cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) Donkey Anti-Goat IgG H&L (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) Donkey Anti-Goat IgG H&L (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab302644 at 1/250 (3.992 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab302644 at 1/250 (3.992 ug/ml) dilution, followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling GFAP with ab302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling GFAP with ab302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) (ab302644)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.