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Goat Recombinant Monoclonal GFAP antibody. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Expected
Tested
Tested
Expected
Expected
Mouse
Not recommended
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/250

Notes

-

Species

Mouse

Dilution info

1/250

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

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Goat Recombinant Monoclonal GFAP antibody. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1034Y-GtIgG

Purification technique

Ion exchange chromatography

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab68428). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on mouse cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

  • Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling GFAP with AB302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Western blot - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: Lane 1: 3.25 seconds
    Lane 2-3: 70 seconds

    All lanes: Western blot - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (AB302644) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Mouse cerebellum tissue lysate at 20 µg

    Lane 3: Rat cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Rabbit anti-Goat IgG (H+L), Peroxidase conjugated at 1/5000 dilution

    Observed band size: 50 kDa

    Exposure time: 70s

  • Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling GFAP with AB302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with AB302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (ab302644)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with AB302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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