Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Goat Recombinant Monoclonal GFAP antibody. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | ICC/IF | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|
Human | Not recommended | Expected | Tested | Tested | Expected | Expected |
Mouse | Not recommended | Tested | Tested | Tested | Tested | Tested |
Rat | Not recommended | Tested | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/250 | Notes - |
Species Mouse | Dilution info 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP, GFAP
Goat Recombinant Monoclonal GFAP antibody. Suitable for ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Glial fibrillary acidic protein, GFAP, GFAP
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR1034Y-GtIgG
Ion exchange chromatography
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab68428). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on mouse cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GFAP with ab302644 at 1/1000 (0.998 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302644 for 30 mins at room temperature, the 2nd antibody Donkey Anti-Goat IgG (HRP) ab205723 was used.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1:2000 dilution.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling GFAP with AB302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 dilution was used as the secondary antibody.
Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: Lane 1: 3.25 seconds
Lane 2-3: 70 seconds
All lanes: Western blot - Anti-GFAP antibody [EPR1034Y] – Goat IgG (Chimeric) (AB302644) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Rat cerebellum tissue lysate at 20 µg
All lanes: Rabbit anti-Goat IgG (H+L), Peroxidase conjugated at 1/5000 dilution
Observed band size: 50 kDa
Exposure time: 70s
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling GFAP with AB302644 at 1/1000 dilution (0.1ug)/ Right (Red) compared with a Goat IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 dilution was used as the secondary antibody.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with AB302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with AB302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com