Rat Recombinant Monoclonal GFAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunocytochemistry - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunocytochemistry-img95954.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303)")
![Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--western-blot-img8299.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71898.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303)")
![Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--flow-cytometry-intracellular-img67970.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303)")
![Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-frozen-sections-img429574.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (AB279303)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC | WB | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected | Expected |
| Mouse | Expected | Tested | Tested | Tested | Expected |
| Rat | Expected | Expected | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Rat Recombinant Monoclonal GFAP antibody. Carrier free. Suitable for IHC-P, ICC, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1034Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab279303 is the carrier free version of Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291.
This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-GFAP antibody [EPR1034Y] ab68428). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunocytochemistry-img95954.jpg "Immunocytochemistry - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Immunocytochemistry - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was developed using Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291, the same antibody clone in a different buffer formulation.
Immunocytochemical analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291 at 1/50 (17 μg/ml) dilution followed by Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 Goat Anti-Rat IgG (Alexa Fluor® 488) pre-adsorbed at 1/1000 dilution (Green). Positive staining on mouse primary astrocytes is observed. The nuclear counterstain was DAPI (Blue).
Counterstain: Anti-GFAP antibody [EPR19996] ab207165 anti-GFAP rabbit mAb at 1/1000 dilution with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) pre-adsorbed at 1/1000 dilution.
Negative controls: Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291 at 1/50 dilution with secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/500 dilution; and Anti-GFAP antibody [EPR19996] ab207165 at 1/1000 dilution with secondary Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 at 1/1000 dilution
![Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--western-blot-img8299.jpg "Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was produced using Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291, the same clone in a different formulation.
The molecular weight observed is consistent with the literature (PMID: 824020, 2294, 6340792).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3.25 seconds; Lane 2, 3: 10 seconds.
All lanes: Western blot - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 49 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71898.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was produced using Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291, the same clone in a different formulation.
IHC image of GFAP staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291, 1μg/ml, for 15 mins at room temperature. A rabbit anti-rat IgG2a, Rabbit Anti-Rat IgG H&L preadsorbed ab102248, was added for 8 mins at room temperature and detected using an HRP conjugated goat anti-rabbit compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.![Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--flow-cytometry-intracellular-img67970.jpg "Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Flow Cytometry (Intracellular) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was produced using Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291, the same clone in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized rat primary neural/glia cells labelling GFAP with Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291 at 1/1000 dilution (0.1μg)/ Right compared with a Rat monoclonal IgG isotype control/ Left.
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-frozen-sections-img429574.jpg "Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291).
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized fresh frozen rat cerebrum tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291 at a 1/200 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at a 1/1000 dilution. The nuclear counterstain was DAPI (blue).
Positive staining on rat cerebrum.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 at a 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-frozen-sections-img429572.jpg "Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291).
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized fresh frozen mouse cerebrum tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) ab279291 at a 1/200 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) secondary antibody at a 1/1000 dilution. The nuclear counterstain was DAPI (blue).
Positive staining on mouse cerebrum.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 at a 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-epr1034y-rat-igg2a-chimeric-bsa-and-azide-free-ab279303--immunohistochemistry-frozen-sections-img429572.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free (ab279303)")
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