AB237844

Anti-GFAP antibody [GFAP/20762076] - BSA and Azide free

Name: Anti-GFAP antibody [GFAP/20762076] - BSA and Azide free (ab237844) | Abcam
Brand: Abcam Limited
SKU: AB237844

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal GFAP antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GFAP aa 100-200.

View Alternative Names

Glial fibrillary acidic protein, GFAP

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [GFAP/20762076] - BSA and Azide free (AB237844)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [GFAP/20762076] - BSA and Azide free (AB237844)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for GFAP using ab224659 at 0.5 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224659)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GFAP/20762076

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Recombinant Fragment Protein within Human GFAP aa 100-200. Database link P14136

style

left-column

Alternative Products

Primary Antibodies

AB207165

Anti-GFAP antibody [EPR19996] - Astrocyte Marker

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR19996] - Astrocyte Marker (AB207165)

Primary Antibodies

AB33922

Anti-GFAP antibody [EP672Y] - Astrocyte Marker

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - Astrocyte Marker (AB33922)

Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

Primary Antibodies

AB218309

Anti-GFAP antibody [EPR1034Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y] - BSA and Azide free (AB218309)

Primary Antibodies

AB220820

Anti-GFAP antibody [EP672Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EP672Y] - BSA and Azide free (AB220820)

Primary Antibodies

AB194325

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194325)

Complementary Products

Assay Kits

AB223867

Human GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Human GFAP ELISA Kit (AB223867)

Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

Primary Antibodies

AB52642

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

Product details

ab237844 is a carrier free version of ab224659.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab224659 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40563509

2025

Positive Effects of (+)-Epicatechin on Traumatic Spinal Cord Injury Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Gonzalez-Ruiz,Rodrigo Mondragón-Lozano,Hermelinda Salgado-Ceballos,Francisco Villarreal,Yuridia Martínez-Meza,Eduardo Meaney,Nayelli Nájera,Guillermo Ceballos

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com