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AB325664

Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric)

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Chicken Monoclonal GFAP antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Glial fibrillary acidic protein, GFAP, Gfap

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of GFAP with ab325664 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the astrocytes in the rat brain. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)

Representative section of formalin fixed, paraffin-embedded rat brain showing staining of GFAP. The sections were stained with ab325664 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the astrocytes in the rat brain. Magnification 400X.

Western blot - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)
  • WB

Supplier Data

Western blot - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (AB325664)

Western blotting of rat brain homogenate with an anti-GFAP mouse mAb and ab325664 antibodies (10 µg/ml). The mouse mAb and ab325664 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous GFAP with similar sensitivity as the anti-GFAP mouse Mab in the rat brain homogenate at 50 kDa.

All lanes:

Western blot - Anti-GFAP antibody [N206A/8] - Chicken IgY (Chimeric) (ab325664) at 10 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 50 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

N206A/8

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Cross-reacts with GFAP-R416W and other GFAP mutant proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N206A/8 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N206A/8 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information Gfap
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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