Anti-GFAP antibody [SP78] - C-terminal (ab227641) is a rabbit monoclonal antibody that is used to detect GFAP in IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139335.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img141842.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136584.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (AB227641)")
Publications
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- Oncotarget 6:22526-522015The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Fazi,Armando Felsani,Luigi Grassi,Anna Moles,Daniel D'Andrea,Nicola Toschi,Daria Sicari,Pasquale De Bonis,Carmelo Anile,Maria Giovanna Guerrisi,Emilia Luca,Maria Giulia Farace,Giulio Maira,Silvia Anna Ciafré,Annunziato MangiolaPubMed 26188123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Anti-GFAP antibody [SP78] - C-terminal (ab227641) is a rabbit monoclonal antibody that is used to detect GFAP in IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP78
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Find all reagents to label astrocytes in our "Astrocytes markers guide".
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139335.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)
Formalin-fixed, paraffin-embedded human astrocytoma tissue stained for GFAP using ab227641 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img141842.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for GFAP using ab227641 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for GFAP using ab227641 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-sp78-c-terminal-astrocyte-marker-ab227641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136584.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [SP78] - C-terminal (ab227641)
Formalin-fixed, paraffin-embedded human eye tissue stained for GFAP using ab227641 at 1/100 dilution in immunohistochemical analysis.
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Product protocols
- Visit the general protocols
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