AB154474

Anti-GFAP antibody [ZRF-1] - Astrocyte Marker

Name: Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474) | Abcam
Brand: Abcam Limited
SKU: AB154474
Price: 475 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal GFAP antibody. Suitable for IP, WB and reacts with Zebrafish, Human samples. Cited in 8 publications.

View Alternative Names

Glial fibrillary acidic protein, GFAP

2 Images

Unknown

Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)

All lanes:

Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474) at 0.5 µg/mL

Lane 1:

Human Brain Homogenate lysate at 10 µg

Lane 2:

Zebrafish Brain Homogenate lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

Predicted band size: 49 kDa

false

Unknown

Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)

Immunoprecipitation Western Blot for ab154474.
All lanes : ab154474 at 0.5 µg/ml.
Lane 1 : Zebrafish Brain Homogenate lysate 10 µg
Lane 2 : GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
Secondary : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.

All lanes:

Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Zrf-1

Isotype

IgG1

Light chain type

lambda

Carrier free

Reacts with

Human, Zebrafish

Applications

IP, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

Near homogeneity as judged by SDS-PAGE. ab154474 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 9:bpae073 PubMed39464854

2024

A reproducible method to study traumatic injury-induced zebrafish brain regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka P Srivastava,Sidharth Bhasin,Sunita S Shankaran,Catherine Roger,Rajesh Ramachandran,Shilpi Minocha

Cell death & disease 15:269 PubMed38627369

2024

Mucopolysaccharidosis type II zebrafish model exhibits early impaired proteasomal-mediated degradation of the axon guidance receptor Dcc.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Manzoli,Lorenzo Badenetti,Matteo Bruzzone,Maria Carla Macario,Michela Rubin,Marco Dal Maschio,Antonella Roveri,Enrico Moro

Nature communications 14:4453 PubMed37488105

2023

In situ assembly of bioresorbable organic bioelectronics in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Hjort,Abdelrazek H Mousa,David Bliman,Muhammad Anwar Shameem,Karin Hellman,Amit Singh Yadav,Peter Ekström,Fredrik Ek,Roger Olsson

International journal of molecular sciences 22: PubMed34072957

2021

Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Ceriani,Kathleen E Whitlock

Frontiers in cell and developmental biology 8:584604 PubMed33163496

2020

Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/β-Catenin Signaling at the Early Wound Healing Stage.

Applications

Unspecified application

Species

Unspecified reactive species

Yeliz Demirci,Gokhan Cucun,Yusuf Kaan Poyraz,Suhaib Mohammed,Guillaume Heger,Irene Papatheodorou,Gunes Ozhan

eLife 9: PubMed32851974

2020

Enteric glia as a source of neural progenitors in adult zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah McCallum,Yuuki Obata,Evangelia Fourli,Stefan Boeing,Christopher J Peddie,Qiling Xu,Stuart Horswell,Robert N Kelsh,Lucy Collinson,David Wilkinson,Carmen Pin,Vassilis Pachnis,Tiffany A Heanue

FASEB journal : official publication of the Federa 32:5132-5142 PubMed29812974

2018

CRISPR/Cas9-based genome engineering of zebrafish using a seamless integration strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Juan-Juan Luo,Wan-Ping Bian,Yi Liu,Hai-Yang Huang,Qian Yin,Xiao-Jun Yang,De-Sheng Pei

Cellular and molecular gastroenterology and hepato 3:484-499 PubMed28462385

2017

Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma.

Applications

Species

Zebrafish

Qiqi Yang,Chuan Yan,Chunyue Yin,Zhiyuan Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFAP antibody [ZRF-1] - Astrocyte Marker

Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)

Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise