Mouse Monoclonal GFAP antibody. Suitable for IP, WB and reacts with Zebrafish, Human samples. Cited in 6 publications.
Images
![Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-zrf-1-astrocyte-marker-ab154474--western-blot-img118389.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)")
![Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-zrf-1-astrocyte-marker-ab154474--immunoprecipitation-img23882.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (AB154474)")
Publications
Filter by
- Nature communications 14:44532023In situ assembly of bioresorbable organic bioelectronics in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Hjort,Abdelrazek H Mousa,David Bliman,Muhammad Anwar Shameem,Karin Hellman,Amit Singh Yadav,Peter Ekström,Fredrik Ek,Roger OlssonPubMed 37488105
- International journal of molecular sciences 22:2021Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Ceriani,Kathleen E WhitlockPubMed 34072957
- Frontiers in cell and developmental biology 8:5846042020Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/β-Catenin Signaling at the Early Wound Healing Stage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeliz Demirci,Gokhan Cucun,Yusuf Kaan Poyraz,Suhaib Mohammed,Guillaume Heger,Irene Papatheodorou,Gunes OzhanPubMed 33163496
- eLife 9:2020Enteric glia as a source of neural progenitors in adult zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah McCallum et. al.PubMed 32851974
- FASEB journal : official publication of the Federa 32:5132-51422018CRISPR/Cas9-based genome engineering of zebrafish using a seamless integration strategy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan-Juan Luo et. al.PubMed 29812974
- Cellular and molecular gastroenterology and hepato 3:484-4992017Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Abcam Recommends
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended |
| Zebrafish | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Mouse Monoclonal GFAP antibody. Suitable for IP, WB and reacts with Zebrafish, Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- Zrf-1
- Light chain type
- lambda
- Concentration
- Purification notes
Near homogeneity as judged by SDS-PAGE. ab154474 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-zrf-1-astrocyte-marker-ab154474--western-blot-img118389.jpg "Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)")
Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)
GFAP Western blot staining using mouse Anti-GFAP antibody
All lanes: Western blot - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474) at 0.5 µg/mL
Lane 1: Human Brain Homogenate lysate at 10 µg
Lane 2: Zebrafish Brain Homogenate lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 49 kDa
![Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474), expandable thumbnail](https://content.abcam.com/products/images/gfap-antibody-zrf-1-astrocyte-marker-ab154474--immunoprecipitation-img23882.jpg "Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)")
Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)
Immunoprecipitation Western Blot for ab154474.
All lanes: ab154474 at 0.5 µg/ml.
Lane 1: Zebrafish Brain Homogenate lysate 10 µg
Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.All lanes: Immunoprecipitation - Anti-GFAP antibody [ZRF-1] - Astrocyte Marker (ab154474)
Predicted band size: 49 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com