Rabbit Recombinant Monoclonal GFAP phospho S13 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, Dot and reacts with Human, Synthetic peptide samples.
Images
![Flow Cytometry - Anti-GFAP (phospho S13) antibody [EPR25281-65] (AB303560), expandable thumbnail](https://content.abcam.com/products/images/gfap-phospho-s13-antibody-epr25281-65-ab303560--flow-cytometry-img402328.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-GFAP (phospho S13) antibody [EPR25281-65] (AB303560)")
![Dot Blot - Anti-GFAP (phospho S13) antibody [EPR25281-65] (AB303560), expandable thumbnail](https://content.abcam.com/products/images/gfap-phospho-s13-antibody-epr25281-65-ab303560--dot-blot-img402395.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-GFAP (phospho S13) antibody [EPR25281-65] (AB303560)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | Dot | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Rabbit Recombinant Monoclonal GFAP phospho S13 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, Dot and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25281-65
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560), expandable thumbnail](https://content.abcam.com/products/images/gfap-phospho-s13-antibody-epr25281-65-ab303560--flow-cytometry-img402328.jpg "Flow Cytometry - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560)")
Flow Cytometry - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) treated with 1ug/ml LPS for 24 hours, then add 100nM Calyculin A for 30 min (Red) / Untreated control (Green) cells labelling GFAP (phospho S13) with ab303560 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Dot Blot - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560), expandable thumbnail](https://content.abcam.com/products/images/gfap-phospho-s13-antibody-epr25281-65-ab303560--dot-blot-img402395.jpg "Dot Blot - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560)")
Dot Blot - Anti-GFAP (phospho S13) antibody [EPR25281-65] (ab303560)
Dot blot analysis of GFAP (phospho S13) using ab303560 at 1:1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution. Exposure time: 180 seconds Blocking and diluting buffer and concentration: 5% NFDM/TBST
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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