AB21061

Anti-GFI1 antibody

Name: Anti-GFI1 antibody (ab21061) | Abcam
Brand: Abcam Limited
SKU: AB21061
Price: 475 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(15 Publications)

Rabbit Polyclonal GFI1 antibody. Suitable for WB and reacts with Human samples. Cited in 15 publications.

View Alternative Names

ZNF163, GFI1, Zinc finger protein Gfi-1, Growth factor independent protein 1, Zinc finger protein 163

1 Images

Western blot - Anti-GFI1 antibody (AB21061)

ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.

All lanes:

Western blot - Anti-GFI1 antibody (ab21061) at 1 µg/mL

Lane 1:

Western blot - THP-1 whole cell lysate (<a href='/en-us/products/cell-lysates/thp-1-whole-cell-lysate-ab7913'>ab7913</a>) at 20 µg

Lane 2:

Western blot - THP-1 whole cell lysate (<a href='/en-us/products/cell-lysates/thp-1-whole-cell-lysate-ab7913'>ab7913</a>) at 20 µg with GFI1 peptide (<a href='/en-us/products/unavailable/gfi1-peptide-ab22461'>ab22461</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)

Predicted band size: 45 kDa

Observed band size: 36 kDa,49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription repressor essential for hematopoiesis (PubMed : 11060035, PubMed : 17197705, PubMed : 17646546, PubMed : 18805794, PubMed : 19164764, PubMed : 20190815, PubMed : 8754800). Functions in a cell-context and development-specific manner (PubMed : 11060035, PubMed : 17197705, PubMed : 17646546, PubMed : 18805794, PubMed : 19164764, PubMed : 20190815, PubMed : 8754800). Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes (PubMed : 11060035, PubMed : 17197705, PubMed : 17646546, PubMed : 18805794, PubMed : 19164764, PubMed : 20190815, PubMed : 8754800). Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed : 16287849). Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development (PubMed : 12778173). Inhibits SPI1 transcriptional activity at macrophage-specific genes, repressing macrophage differentiation of myeloid progenitor cells and promoting granulocyte commitment (By similarity). Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling (By similarity). Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA (PubMed : 20547752). Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth (PubMed : 19026687). Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters (PubMed : 16287849). Required for the maintenance of inner ear hair cells (By similarity). In addition to its role in transcription, acts as a substrate adapter for PRMT1 in the DNA damage response : facilitates the recognition of TP53BP1 and MRE11 substrates by PRMT1, promoting their methylation and the DNA damage response (PubMed : 29651020).

See full target information GFI1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 21:631-647 PubMed36976323

2023

Disruption of the MYC Superenhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

William M Yashar,Brittany M Curtiss,Daniel J Coleman,Jake VanCampen,Garth Kong,Jommel Macaraeg,Joseph Estabrook,Emek Demir,Nicola Long,Daniel Bottomly,Shannon K McWeeney,Jeffrey W Tyner,Brian J Druker,Julia E Maxson,Theodore P Braun

Frontiers in cell and developmental biology 10:1016367 PubMed36420143

2022

Repurposing the lineage-determining transcription factor Atoh1 without redistributing its genomic binding sites.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Costa,Lynn M Powell,Mattias Malaguti,Abdenour Soufi,Sally Lowell,Andrew P Jarman

International journal of molecular medicine 48: PubMed34368875

2021

GFI1 promotes the proliferation and migration of esophageal squamous cell carcinoma cells through the inhibition of SOCS1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Huang,Ran Ruan,Yanxin Fang,Kaiming Wu,Long Yao,Renquan Zhang,Wei He

Oncology letters 22:521 PubMed34025788

2021

GFI-1 overexpression promotes cell proliferation and apoptosis resistance in mycosis fungoides by repressing Bax and P21.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoguang Gu,Yimeng Wang,Chunlei Zhang,Yongsheng Liu

Molecular therapy. Nucleic acids 23:418-430 PubMed33473327

2021

Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Meixiong Cheng,Yi Zeng,Tian Zhang,Min Xu,Zhili Li,Yaqiu Wu

The EMBO journal :e104983 PubMed33103827

2020

Interactions between lineage-associated transcription factors govern haematopoietic progenitor states.

Applications

Unspecified application

Species

Unspecified reactive species

Iwo Kucinski,Nicola K Wilson,Rebecca Hannah,Sarah J Kinston,Pierre Cauchy,Aurelie Lenaerts,Rudolf Grosschedl,Berthold Göttgens

Molecular carcinogenesis 59:679-690 PubMed31912614

2020

Loss of myeloid-specific lamin A/C drives lung metastasis through Gfi-1 and C/EBPε-mediated granulocytic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Ishii,Woo-Yong Park,Jaeyoung So,Skyler Kuhn,Vishal N Koparde,Yanli Pang,Tim F Greten,M Christine Hollander,Li Yang

Nature communications 10:332 PubMed30659187

2019

Lsd1 as a therapeutic target in Gfi1-activated medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Lee,Vasilisa A Rudneva,Serap Erkek,Marc Zapatka,Lianne Q Chau,Silvia K Tacheva-Grigorova,Alexandra Garancher,Jessica M Rusert,Ozlem Aksoy,Robin Lea,Helai P Mohammad,Jianxun Wang,William A Weiss,H Leighton Grimes,Stefan M Pfister,Paul A Northcott,Robert J Wechsler-Reya

Blood advances 3:33-46 PubMed30617215

2019

microRNA-22 promotes megakaryocyte differentiation through repression of its target, .

Applications

Unspecified application

Species

Unspecified reactive species

Cary N Weiss,Keisuke Ito

Frontiers in endocrinology 9:344 PubMed30008697

2018

XRK3F2 Inhibition of p62-ZZ Domain Signaling Rescues Myeloma-Induced GFI1-Driven Epigenetic Repression of the Gene in Pre-osteoblasts to Overcome Differentiation Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Juraj Adamik,Rebecca Silbermann,Silvia Marino,Quanhong Sun,Judith L Anderson,Dan Zhou,Xiang-Qun Xie,G David Roodman,Deborah L Galson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFI1 antibody

Western blot - Anti-GFI1 antibody (AB21061)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise