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AB173529

Anti-GFM1 antibody [EPR12263-33]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal GFM1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

EFG, EFG1, GFM, GFM1, EF-Gmt, Elongation factor G1, mEF-G 1, hEFG1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GFM1 antibody [EPR12263-33] (AB173529)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFM1 antibody [EPR12263-33] (AB173529)

Immunofluorescence analysis of HeLa cells labeling GFM1 with ab173529 at a 1/50 dilution.

Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] (AB173529)
  • IP

Supplier Data

Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] (AB173529)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate : ab173529 at a 1/10 dilution was used for immunoprecipitate GFM1.

All lanes:

Immunoprecipitation - Anti-GFM1 antibody [EPR12263-33] (ab173529)

Predicted band size: 83 kDa

false

Western blot - Anti-GFM1 antibody [EPR12263-33] (AB173529)
  • WB

Supplier Data

Western blot - Anti-GFM1 antibody [EPR12263-33] (AB173529)

All lanes:

Western blot - Anti-GFM1 antibody [EPR12263-33] (ab173529) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

NCI-H460 cell lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 83 kDa

false

  • Carrier free

    Anti-GFM1 antibody [EPR12263-33] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12263-33

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
See full target information GFM1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40002401

2025

Polydatin and Nicotinamide Prevent Iron Accumulation and Lipid Peroxidation in Cellular Models of Mitochondrial Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José Antonio Sánchez-Alcázar

Journal of inherited metabolic disease 46:261-272 PubMed36564894

2023

Pathogenic variants of the coenzyme A biosynthesis-associated enzyme phosphopantothenoylcysteine decarboxylase cause autosomal-recessive dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Bravo-Alonso,Matías Morin,Laura Arribas-Carreira,Mar Álvarez,Consuelo Pedrón-Giner,Lucia Soletto,Carlos Santolaria,Santiago Ramón-Maiques,Magdalena Ugarte,Pilar Rodríguez-Pombo,Joaquín Ariño,Miguel Ángel Moreno-Pelayo,Belén Pérez

Orphanet journal of rare diseases 17:204 PubMed35581596

2022

UPR activation improves pathological alterations in cellular models of mitochondrial diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Suárez-Rivero,Carmen J Pastor-Maldonado,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,Irene Villalón-García,Marta Talaverón-Rey,Alejandra Suárez-Carrillo,Manuel Munuera-Cabeza,Diana Reche-López,Paula Cilleros-Holgado,Rocío Piñero-Perez,José A Sánchez-Alcázar

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22091 PubMed34919756

2021

Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Molina-Berenguer,Ferran Vila-Julià,Sandra Pérez-Ramos,Maria Teresa Salcedo-Allende,Yolanda Cámara,Javier Torres-Torronteras,Ramon Martí

Journal of clinical medicine 8: PubMed31683770

2019

Genes and Variants Underlying Human Congenital Lactic Acidosis-From Genetics to Personalized Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Bravo-Alonso,Rosa Navarrete,Ana Isabel Vega,Pedro Ruíz-Sala,María Teresa García Silva,Elena Martín-Hernández,Pilar Quijada-Fraile,Amaya Belanger-Quintana,Sinziana Stanescu,María Bueno,Isidro Vitoria,Laura Toledo,María Luz Couce,Inmaculada García-Jiménez,Ricardo Ramos-Ruiz,Miguel Ángel Martín,Lourdes R Desviat,Magdalena Ugarte,Celia Pérez-Cerdá,Begoña Merinero,Belén Pérez,Pilar Rodríguez-Pombo

Physiological reports 6:e13893 PubMed30369085

2018

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Yokokawa,Kohei Kido,Tadashi Suga,Tadao Isaka,Tatsuya Hayashi,Satoshi Fujita

Frontiers in genetics 6:102 PubMed25852744

2015

Long-term survival in a child with severe encephalopathy, multiple respiratory chain deficiency and GFM1 mutations.

Applications

WB

Species

Unspecified reactive species

Sara Brito,Kyle Thompson,Jaume Campistol,Jaime Colomer,Steven A Hardy,Langping He,Ana Fernández-Marmiesse,Lourdes Palacios,Cristina Jou,Cecilia Jiménez-Mallebrera,Judith Armstrong,Raquel Montero,Rafael Artuch,Christin Tischner,Tina Wenz,Robert McFarland,Robert W Taylor
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com