Rabbit Recombinant Monoclonal GFM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--western-blot-img116048.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102729.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43684.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (AB249664)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
Alternative names
EFG, EFG1, GFM, GFM1, EF-Gmt, Elongation factor G1, mEF-G 1, hEFG1
Recommended products
Rabbit Recombinant Monoclonal GFM1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12263
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249664 is the carrier-free version of Anti-GFM1 antibody [EPR12263] ab171945.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--western-blot-img116048.jpg "Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)")
Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
This data was developed using Anti-GFM1 antibody [EPR12263] ab171945, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GFM1 antibody [EPR12263] (Anti-GFM1 antibody [EPR12263] ab171945) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: NCI 460 cell line lysate at 10 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg
Lane 4: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102729.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
This data was developed using Anti-GFM1 antibody [EPR12263] ab171945, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling GFM1 with Anti-GFM1 antibody [EPR12263] ab171945 at 1/50 Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664), expandable thumbnail](https://content.abcam.com/products/images/gfm1-antibody-epr12263-bsa-and-azide-free-ab249664--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43684.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
This data was developed using Anti-GFM1 antibody [EPR12263] ab171945, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling GFM1 with Anti-GFM1 antibody [EPR12263] ab171945 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com