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AB229463

Anti-GFM2 antibody

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(1 Publication)

Rabbit Polyclonal GFM2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GFM2.

View Alternative Names

EFG2, MSTP027, GFM2, RRF2mt, Elongation factor G2, EF-G2mt, mEF-G 2, hEFG2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GFM2 antibody (AB229463)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFM2 antibody (AB229463)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GFM2 (green) using ab229463 at 1/500 dilution in ICC/IF. Cells were fixed with 2% paraformaldehyde/culture medium at 37oC for 30 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM2 antibody (AB229463)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM2 antibody (AB229463)

Paraffin-embedded mouse testis tissue stained for GFM2 using ab229463 at 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-GFM2 antibody (AB229463)
  • WB

Supplier Data

Western blot - Anti-GFM2 antibody (AB229463)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-GFM2 antibody (ab229463) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GFM2. The exact immunogen used to generate this antibody is proprietary information.

Q969S9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis (PubMed : 19716793, PubMed : 33878294). Acts in collaboration with MRRF (PubMed : 19716793, PubMed : 33878294). Promotes mitochondrial ribosome recycling by dissolution of intersubunit contacts (PubMed : 33878294). GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit (PubMed : 19716793). Not involved in the GTP-dependent ribosomal translocation step during translation elongation (PubMed : 19716793).
See full target information GFM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Chemical Society 146:12410-12422 PubMed38669207

2024

Programmable RNA Loading of Extracellular Vesicles with Toehold-Release Purification.

Applications

Unspecified application

Species

Unspecified reactive species

Mette Galsgaard Malle,Ping Song,Philipp M G Löffler,Nazmie Kalisi,Yan Yan,Julián Valero,Stefan Vogel,Jørgen Kjems
View all publications
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Product promise

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