Anti-GFP antibody [1F1]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [1F1] (AB315212)

Immunofluorescent analysis of transfected HEK293 cells transfected with a GFP construct and stained with ab315212, dilution 1 : 1,000, in red. The blue is Hoechst staining of nuclear DNA. The ab315212 antibody binds to GFP protein expressed only in transfected cells, and as a result cells are appeared in orange-golden color.

• WB

Supplier Data

Western blot - Anti-GFP antibody [1F1] (AB315212)

Western blot analysis of transfected and control HEK293 cell lysates using ab315212, in green, dilution 1 : 1,000 : [1] protein standard, [2] Control, non-transfected cells, [3] cells transfected with an mCherry red fluorescent protein construct and [4] cells transfected with GFP construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not bind to mCherry. The same blot was simultaneously probed with chicken pAb to HSP60, CPCA-HSP60, dilution 1 : 10,000, in red. The single band at 60kDa represents the HSP60 protein expressed in all preparations

All lanes:

Western blot - Anti-GFP antibody [1F1] (ab315212) at 1/1000 dilution

Lane 2:

non-transfected HEK293 cell lysate

Lane 3:

HEK293 cell lysate transfected with an mCherry red fluorescent protein construct

Lane 4:

HEK293 cell lysate transfected with GFP construct

Observed band size: 27 kDa,60 kDa

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