Anti-GFP antibody [3H9]

7 product images

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  Western blot - Anti-GFP antibody [3H9] (ab252881)

  Blocking and Diluting buffer: 5% NFDM/TBST
  

  Exposure time: 3 seconds

  All lanes: Western blot - Anti-GFP antibody [3H9] (ab252881) at 1/5000 dilution

  Lane 1: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

  Lane 2: HEK-293T transfected with GFP expression vector, whole cell lysate at 10 µg

  Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

  Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/100000 dilution

  Predicted band size: 27 kDa

  Observed band size: 27 kDa

  Exposure time: 3s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (ab252881)

  Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP construct (A) labeling GFP with ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). No staining observed on HEK-293T cells (B). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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  Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [3H9] (ab252881)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for GFP (red) using ab252881 at 1/100 dilution (10.78 μg/ml), followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with GFP only plasmid. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (ab252881)

  Immunohistochemical analysis of paraffin-embedded human tonsil section labeling GFP with ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.
  

  Negative control: no staining on human tonsil.

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (ab252881)

  Immunohistochemical analysis of paraffin-embedded mouse spleen section labeling GFP with ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.
  

  Negative control: no staining on mouse spleen.

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (ab252881)

  Immunohistochemical analysis of paraffin-embedded rat liver section labeling GFP with ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.
  

  Negative control: no staining on rat liver.

  Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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  This image is courtesy of a customer review submitted by Prashant Rai

  Immunocytochemistry - Anti-GFP antibody [3H9] (ab252881)

  Immunocytochemistry analysis of paraformaldehyde-fixed 1x PBS permeabilized porcine fetal fibroblast cells staining with ab252881. Secondary antibody was Alexa Fluor™ 594 IgG(H+L). Samples were incubated with the primary antibody with PBS and BSA for 12 hours at 4°C. Blocking was done using 1% BSA for 1 hour at 25°C.