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Rat Recombinant Monoclonal GFP antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Tag samples.

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Images

Western blot - Anti-GFP antibody [3H9] - BSA and Azide free (AB255886), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (AB255886), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [3H9] - BSA and Azide free (AB255886), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (AB255886), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (AB255886), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFWBIHC-P
Tag
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species
Tag
Dilution info
-
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

Recommended products

Rat Recombinant Monoclonal GFP antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Tag samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
3H9
Purification technique
Affinity purification Protein A
Specificity

This mAb recognizes eGFP, wild-type GFP, YFP and CFP.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255886 is the carrier-free version of Anti-GFP antibody [3H9] ab252881.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Western blot - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer: 5% NFDM/TBST

    Exposure time: 3 seconds

    All lanes: Western blot - Anti-GFP antibody [3H9] (Anti-GFP antibody [3H9] ab252881) at 1/5000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with GFP expression vector, whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/100000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP construct (A) labeling GFP with Anti-GFP antibody [3H9] ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). No staining observed on HEK-293T cells (B). The section was incubated with Anti-GFP antibody [3H9] ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for GFP (red) using Anti-GFP antibody [3H9] ab252881 at 1/100 dilution (10.78 μg/ml), followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with GFP only plasmid. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil section labeling GFP with Anti-GFP antibody [3H9] ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with Anti-GFP antibody [3H9] ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on human tonsil.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen section labeling GFP with Anti-GFP antibody [3H9] ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with Anti-GFP antibody [3H9] ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on mouse spleen.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] - BSA and Azide free (ab255886)

    This data was developed using Anti-GFP antibody [3H9] ab252881 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver section labeling GFP with Anti-GFP antibody [3H9] ab252881 at 1/2000 dilution (0.539 μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). The section was incubated with Anti-GFP antibody [3H9] ab252881 for 30 mins at room temperature. Counterstained with Hematoxylin.

    Negative control: no staining on rat liver.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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