Anti-GFP antibody [9F9.F9]

4 product images

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  Grant, M.P. et al PLoS One. 2015 Aug 28;10(8):e0136702. doi: 10.1371/journal.pone.0136702. eCollection 2015

  Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)

  TIRFM responses to prolonged stimulation with 10 mM Ca²⁺

  C. Cells expressing BS-wt were stimulated with 0.5 or 10 mM Ca²⁺ for 60 min, 37°C, followed by fixation, permeabilization, and staining with anti-GFP antibody (secondary antibody tagged with Alexa-568). Confocal images of SEP and anti-GFP fluorescence are shown along with the merged image. D. Experiment as in C for cells expressing BS-5A mutant.

  HEK-293 cells were seeded on glass coverslips and transiently transfected with BS-wt or BS-5A cDNAs. Cells were incubated in DMEM containing 0.5 or 10 mM Ca²⁺ for 1 hr at 37°C, 5% CO₂. Following incubation, cells were immediately fixed in 4% paraformaldehyde for 10 min, RT, permeabilized with 0.2% Triton X-100 (10 min), blocked with 5% serum and 0.1% BSA then incubated with monoclonal anti-GFP antibody (Abcam), followed by goat anti-mouse secondary antibody conjugated to Alexa-568.

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  Western blot - Anti-GFP antibody [9F9.F9] (ab1218)

  All lanes: Western blot - Anti-GFP antibody [9F9.F9] (ab1218) at 1 mg/mL

  Lane 1: HeLa lysate 50ng with BLOTTO, overnight at 4°C

  Lane 2: HeLa lysate 100ng with BLOTTO, overnight at 4°C

  Lane 3: HeLa lysate 500ng with BLOTTO, overnight at 4°C

  Secondary

  All lanes: IRDye® 800 conjugated Goat anti-mouse IgG (H+L), 45 minutes at room temperature at 1/2500 dilution

  Predicted band size: 27 kDa

  Observed band size: 27 kDa

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  This image is courtesy of an anonymous customer review

  Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (ab1218)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling GFP with ab1218 at 1/100. Cells were fixed with Formaldehyde and permeabilised with 0.2% Triton100 (permeablized after primary incubation). A good anti-mouse Texas Red^® at 1/1000 was used as the secondary antibody.

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  Image courtesy of an anonymous customer review

  Western blot - Anti-GFP antibody [9F9.F9] (ab1218)

  All lanes: Western blot - Anti-GFP antibody [9F9.F9] (ab1218) at 1/1000 dilution

  Lane 1: J774 at 20 µg with Milk, 2 hours at 25°C

  Lane 2: J774+pEGFP-N1 at 20 µg with Milk, 2 hours at 25°C

  Secondary

  All lanes: Goat anti-mouse HRP at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 27 kDa

  Observed band size: 26 kDa

  Exposure time: 1min