Anti-GFP antibody [9F9.F9]

• ICC/IF

Project

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (AB1218)

TIRFM responses to prolonged stimulation with 10 mM Ca²⁺

C. Cells expressing BS-wt were stimulated with 0.5 or 10 mM Ca²⁺ for 60 min, 37°C, followed by fixation, permeabilization, and staining with anti-GFP antibody (secondary antibody tagged with Alexa-568). Confocal images of SEP and anti-GFP fluorescence are shown along with the merged image. D. Experiment as in C for cells expressing BS-5A mutant.

HEK-293 cells were seeded on glass coverslips and transiently transfected with BS-wt or BS-5A cDNAs. Cells were incubated in DMEM containing 0.5 or 10 mM Ca²⁺ for 1 hr at 37°C, 5% CO₂. Following incubation, cells were immediately fixed in 4% paraformaldehyde for 10 min, RT, permeabilized with 0.2% Triton X-100 (10 min), blocked with 5% serum and 0.1% BSA then incubated with monoclonal anti-GFP antibody (Abcam), followed by goat anti-mouse secondary antibody conjugated to Alexa-568.

Grant, M.P. et al PLoS One. 2015 Aug 28;10(8):e0136702. doi: 10.1371/journal.pone.0136702. eCollection 2015

• ICC/IF

AbReview38905****

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (AB1218)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling GFP with ab1218 at 1/100. Cells were fixed with Formaldehyde and permeabilised with 0.2% Triton100 (permeablized after primary incubation). A good anti-mouse Texas Red^® at 1/1000 was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• WB

AbReview32761****

Western blot - Anti-GFP antibody [9F9.F9] (AB1218)

All lanes:

Western blot - Anti-GFP antibody [9F9.F9] (ab1218) at 1/1000 dilution

Lane 1:

J774 at 20 µg

Lane 2:

J774+pEGFP-N1 at 20 µg

Secondary

All lanes:

Goat anti-mouse HRP at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 26 kDa

true

Exposure time: 1min

Image courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-GFP antibody [9F9.F9] (AB1218)

All lanes:

Western blot - Anti-GFP antibody [9F9.F9] (ab1218) at 1 mg/mL

Lane 1:

HeLa lysate 50ng

Lane 2:

HeLa lysate 100ng

Lane 3:

HeLa lysate 500ng

Secondary

All lanes:

IRDye® 800 conjugated Goat anti-mouse IgG (H+L), 45 minutes at room temperature at 1/2500 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false