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Chicken anti-GFP antibody ab13970 is a chicken polyclonal antibody that is used in GFP western blotting and immunofluorescence.

Tried and trusted by researchers since 2004
Anti-GFP antibody ab13970 is cited in over 3820 publications
Same trusted quality, New lower price!


Images

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.16% Sodium phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Tag
Tested
Tested

Tested
Tested

Species

Tag

Dilution info

1/5000

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/2000

Notes

Used at a dilution of 1/2000 for 1 hr (see Abreview for further information).

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

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Chicken anti-GFP antibody ab13970 is a chicken polyclonal antibody that is used in GFP western blotting and immunofluorescence.

Tried and trusted by researchers since 2004
Anti-GFP antibody ab13970 is cited in over 3820 publications
Same trusted quality, New lower price!

Alternative names

Key facts

Isotype

IgY

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212
Purity

IgY fraction

Specificity

Our GFP antibody does cross-react with the many fluorescent proteins that are derived from the jellyfish Aequorea victoria. These are all proteins that differ from the original GFP by just a few point mutations (EGFP, YFP, mVenus, CFP, BFP etc.).

Concentration
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Purification notes

Sterile filtered.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

GFP serves as a marker due to its ability to emit green fluorescence without requiring additional substrates or cofactors. GFP does not function within complexes like other proteins but acts as a standalone tool to monitor physiological processes. Scientists utilize techniques such as Western blot ELISA and microscopy along with GFP to track and quantify proteins inside living cells. Anti-GFP antibodies can detect GFP fusion proteins in various applications providing valuable insights into protein behavior and allowing robust assays involving GFP.

Activity summary

GFP also known as Green Fluorescent Protein acts as a bioluminescent marker derived from the jellyfish Aequorea victoria. GFP is popular in molecular biology for its fluorescence properties making it useful for visualizing proteins. This protein has a molecular weight of approximately 27 kDa. Researchers and scientists often express GFP in various organisms as a luminescent tag helping them observe protein expression localization and interaction within cells. GFP tagging involves the fusion of GFP to a protein of interest enabling the study of the protein's function and dynamics without affecting the host cell.

Pathways

GFP itself does not participate actively in traditional biochemical or signaling pathways. Instead it enables visual tracking within pathways. Researchers utilize GFP to study pathways like MAPK/ERK and PI3K/AKT where they track proteins related to these pathways using GFP tagging. For instance fusing GFP with proteins like ERK1/2 allows tracking phosphorylation events and signal transduction in living cells leading to better understanding of cellular responses to different stimuli.

Associated diseases and disorders

Researchers use GFP as a model to study gene expression and protein interactions under disease conditions. For example in neurological disorders GFP helps visualize neuronal pathways and protein aggregation processes. By tagging proteins such as amyloid precursor protein (APP) or tau with GFP scientists can study their role in Alzheimer's disease progression. Similarly GFP facilitates the investigation of cancer pathways allowing visualization of tumor-related proteins and helping researchers study how cancer cells grow and invade tissues supporting cancer research and therapy development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-GFP antibody (ab13970), expandable thumbnail
    Image courtesy of an anonymous customer review.

    Western blot - Anti-GFP antibody (ab13970)

    All lanes: Western blot - Anti-GFP antibody (AB13970) at 1/1000 dilution

    Lane 1: Whole cell lysate prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. at 25 µg

    Lane 2: Whole cell lysate prepared from HeLa cells. at 25 µg

    Secondary

    All lanes: IRDye 800CW conjugated goat anti-chicken polyclonal at 1/15000 dilution

    Predicted band size: 27 kDa

  • Western blot - Anti-GFP antibody (ab13970), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-GFP antibody (ab13970)

    Gel Running Conditions: Reduced Denaturing (15% PAGE)

    Detection method: Fluorescent Secondary Antibodies

    All lanes: Western blot - Anti-GFP antibody (AB13970) at 1/2000 dilution

    Lane 1: 3 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg

    Lane 2: 2 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg

    Lane 3: 1 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (AB150176) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 27 kDa

    Observed band size: 25 kDa

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Radbod Darabi

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)

    ab13970 staining GFP + tumor in mouse muscle cells by ICC/IF.

    Cells were formaldehyde fixed and blocked with 3% BSA for 1 hour at 24°C prior to incubation with the primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat anti-chicken was used as the secondary.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970), expandable thumbnail
    This image is courtesy of a customer review submitted by Christophe Lachaud

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)

    ab13970 staining GFP in U-2 OS (Human bone osteosarcoma epithelial cell line) cells by ICC/IF.

    Cells were paraformaldehyde fixed, permeabilized with 0.5% triton and blocked with 2% antibody dilution buffer for 2 hours. Cells were incubated with the primary antibody (1/1000) for 1 hour at 25°C. An undiluted Alexa Fluor® 488 conjugated Goat anti-chicken polyclonal was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)

    Transgenic mice expressing GFP selectively in lamina II of the spinal cord.

    In the right panels, note the correspondance between the green (rabbit anti-GFP) and red signals (chicken anti-GFP from Abcam) indicating that these two antibody preparations recognized the same gene product. The secondary antibody used with ab13970 was an FITC-labeled goat anti-chicken

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)

    ab13970 staining GFP in GFP-transfected NIH/3T3 (Mouse embryo fibroblast cell line) cells.

    The cells were fixed with 4% formaldehyde (10 minutes) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab13970 at 1/2000 dilution overnight at +4°C followed by incubation with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed (ab150173), for 1 hour, at 1 μg/ml.
    Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH/3T3 cells, the cells upon which ab13970 was applied gave a stronger signal in the 488 channel, indicating that ab13970 is binding to GFP and therefore eliciting signal amplification.
    ab13970 was also applied to non-GFP-transfected NIH/3T3 cells, which produced no positive staining, indicating specificity for GFP.

    Nuclear DNA was labeled with 1.43 μM DAPI (blue).

  • Western blot - Anti-GFP antibody (ab13970), expandable thumbnail

    Western blot - Anti-GFP antibody (ab13970)

    Western blot of transgenic mouse spinal cords showing that the rabbit anti-GFP (lane 1) and the chicken anti-GFP (Abcam; lane 2) recognize a band at the same molecular weight.

    Lane 1: Rabbit anti-GFP

    Lane 2: Western blot - Anti-GFP antibody (AB13970)

    All lanes: Transgenic mouse spinal cords

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970), expandable thumbnail
    Image courtesy of a customer review submitted by Dr Francois Daubeuf.

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab13970)

    Immunocytochemical/ Immunoflurescence analysis of cytospined HEK-293 cells (Human epithelial cell line from embryonic kidney) transfected with GFP, labeling GFP with ab13970 at 1/200 incubated for 16 hours at 4°C with 1% BSA in PBS. Secondary used was a donkey anti-chicken polyclonal DyLight® 594 at 1/500.

    GFP is shown in red (DyLight® 594).

    Nuclei are counterstained in blue (DAPI).

    The left panel shows HEK-293 cells transfected with GFP and the right panel shows non-transfected HEK-293 cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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