Chicken Polyclonal GFP antibody. Suitable for ICC/IF, WB and reacts with Tag samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Aequorea victoria GFP.
Images
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Tag | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/10000 | Notes - |
Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Alternative names
Green fluorescent protein, GFP
Recommended products
Chicken Polyclonal GFP antibody. Suitable for ICC/IF, WB and reacts with Tag samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Aequorea victoria GFP.
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were immunoaffinity purified using the native GFP immobilized on a solid phase.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-GFP antibody (ab307275)
GFP Western blot staining using chicken Anti-GFP antibody
CHO cells were transfected with pcDNA3 GFP (as CHO-GFP). The whole cell lysates were loaded as 2ug and 0.2ug per lane then incubated with Chicken-anti GFP (ab307275) at 1/10000 dilution (0.1ug/ml) for 1 hour at room temperature and followed with Goat anti-Chicken HRP conjugated secondary at 1/10000 dilution (0.1ug/ml) incubation at 1 hour at room temperature.
All lanes: Western blot - Anti-GFP antibody (ab307275) at 1/10000 dilution
Lane 1: CHO cells transfected with pcDNA3 GFP (as CHO-GFP) at 2 µg
Lane 2: CHO cells transfected with pcDNA3 GFP (as CHO-GFP) at 0.2 µg
SecondaryAll lanes: Goat anti-Chicken HRP conjugated secondary at 1/10000 dilution
Observed band size: 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (ab307275)
Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with GFP constructs (CHO-GFP) labelling GFP with ab307275 at 1/100 dilution (10ug/ml), followed by a Goat Anti-Chicken IgY (H&L), Alexa Fluor 594 conjugated secondary antibody at 1/400 dilution (5 ug/ml) (Red). Images show GFP (Green) and nuclear counterstain with DAPI (Blue). Images show parallel staining in parental CHO cell line.
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Product protocols
- Visit the general protocols
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