Anti-GFP antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB307275)

Secondary only antibody control : Cells labeled with only secondary antibody Goat anti-chicken IgY (H&L) - AF594 at 1/400 dilution (5 µg/ml).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB307275)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with GFP constructs (CHO-GFP) labelling GFP with ab307275 at 1/100 dilution (10ug/ml), followed by a Goat Anti-Chicken IgY (H&L), Alexa Fluor 594 conjugated secondary antibody at 1/400 dilution (5 ug/ml) (Red). Images show GFP (Green) and nuclear counterstain with DAPI (Blue). Images show parallel staining in parental CHO cell line.

• WB

Supplier Data

Western blot - Anti-GFP antibody (AB307275)

CHO cells were transfected with pcDNA3 GFP (as CHO-GFP). The whole cell lysates were loaded as 2ug and 0.2ug per lane then incubated with Chicken-anti GFP (ab307275) at 1/10000 dilution (0.1ug/ml) for 1 hour at room temperature and followed with Goat anti-Chicken HRP conjugated secondary at 1/10000 dilution (0.1ug/ml) incubation at 1 hour at room temperature.

All lanes:

Western blot - Anti-GFP antibody (ab307275) at 1/10000 dilution

Lane 1:

CHO cells transfected with pcDNA3 GFP (as CHO-GFP) at 2 µg

Lane 2:

CHO cells transfected with pcDNA3 GFP (as CHO-GFP) at 0.2 µg

Secondary

All lanes:

Goat anti-Chicken HRP conjugated secondary at 1/10000 dilution

Observed band size: 28 kDa

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