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AB307276

Anti-GFP antibody

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Rabbit Polyclonal GFP antibody. Suitable for ICC/IF, WB.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB307276)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB307276)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with GFP constructs (CHO-GFP) labelling GFP with ab307276 at 1/100 dilution (10ug/ml), followed by a Goat Anti-Rabbit IgG (H&L), Alexa Fluor 594 conjugated secondary antibody at 1/400 dilution (5 ug/ml) (Red). Images show GFP (Green), and nucleaer counterstain with DAPI (Blue). Bottom panel images show parallel staining in parental CHO cell line.

Western blot - Anti-GFP antibody (AB307276)
  • WB

Supplier Data

Western blot - Anti-GFP antibody (AB307276)

CHO cells were transfected with pcDNA3-GFP (CHO-GFP). The whole cell lysates were loaded as 2ug and 0.2ug per lane then incubated with Rabbit-anti GFP (ab307276) at 1/10000 dilution (0.1ug/ml) for 1 hour at room temperature and followed with Goat anti-Rabbit HRP conjugated secondary at 1/10000 dilution (0.1ug/ml) incubation at 1 hour at room temperature.

All lanes:

Western blot - Anti-GFP antibody (ab307276) at 1/10000 dilution

Lane 1:

CHO cells transfected with pcDNA3-GFP (CHO-GFP) at 2 µg

Lane 2:

CHO cells transfected with pcDNA3-GFP (CHO-GFP) at 0.2 µg

Secondary

All lanes:

Goat anti-Rabbit HRP conjugated secondary at 1/10000 dilution

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was immunoaffinity purified off a native GFP containing immunosorbent
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com