AB5449

Anti-GFP antibody

Name: Anti-GFP antibody (ab5449) | Abcam
Brand: Abcam Limited
SKU: AB5449
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(21 Publications)

Goat Polyclonal GFP antibody. Suitable for IP, WB.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

2 Images

Unknown

Western blot - Anti-GFP antibody (AB5449)

Lane 1 : parental YTS cells (negative control)
Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD)

KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x10⁶ cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33 : 1084).

Lane 1 : parental YTS cells (negative control) Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD) KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33 : 1084).

All lanes:

Western blot - Anti-GFP antibody (ab5449)

Predicted band size: 27 kDa

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Western blot - Anti-GFP antibody (AB5449)

5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of :
Lane 1 : 1/2500
Lane 2 : 1/5000
Lane 3 : 1/10,000
Lane 4 : 1/20,000

5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of : Lane 1 : 1/2500 Lane 2 : 1/5000 Lane 3 : 1/10,000 Lane 4 : 1/20,000

All lanes:

Western blot - Anti-GFP antibody (ab5449)

Predicted band size: 27 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": " for 10^6 Cells", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" } } }

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Product details

Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. This antibody is available in an affinity purified form as ab5450.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 80:103521 PubMed39908862

2025

ROS anchor PAMPs-mediated extracellular HMGB1 self-association and its dimerization enhances pro-inflammatory signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Man Sup Kwak,Myeonggil Han,Yong Joon Lee,Seoyeon Choi,Jeonghwa Kim,In Ho Park,Jeon-Soo Shin

Communications biology 8:2 PubMed39747573

2025

NLS-binding deficient Kapβ2 reduces neurotoxicity via selective interaction with C9orf72-ALS/FTD dipeptide repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin M Kim,Amandeep Girdhar,Maria E Cicardi,Vaishnavi Kankate,Miyuki Hayashi,Ruoyu Yang,Jenny L Carey,Charlotte M Fare,James Shorter,Gino Cingolani,Davide Trotti,Lin Guo

Science advances 10:eadl0788 PubMed38517962

2024

FOXL2 interaction with different binding partners regulates the dynamics of ovarian development.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Migale,Michelle Neumann,Richard Mitter,Mahmoud-Reza Rafiee,Sophie Wood,Jessica Olsen,Robin Lovell-Badge

Molecular therapy : the journal of the American Society of Gene Therapy 32:1096-1109 PubMed38291756

2024

Knockdown of calpain1 in lumbar motoneurons reduces spasticity after spinal cord injury in adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Marjorie Kerzonkuf,Jérémy Verneuil,Cécile Brocard,Nejada Dingu,Virginie Trouplin,Jose Jorge Ramirez Franco,Marc Bartoli,Frédéric Brocard,Hélène Bras

International journal of molecular sciences 23: PubMed35742810

2022

Structural and Functional Insights into CP2c Transcription Factor Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Han Son,Min Young Kim,Eunbi Jo,Vladimir N Uversky,Chul Geun Kim

Journal of neurophysiology 126:1978-1990 PubMed34669520

2021

Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy W Chopek,Ying Zhang,Robert M Brownstone

Neuron 109:2847-2863.e11 PubMed34407390

2021

Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.

Applications

Unspecified application

Species

Unspecified reactive species

Sisu Han,Satoshi Okawa,Grey Atteridge Wilkinson,Hussein Ghazale,Lata Adnani,Rajiv Dixit,Ligia Tavares,Imrul Faisal,Matthew J Brooks,Veronique Cortay,Dawn Zinyk,Adam Sivitilli,Saiqun Li,Faizan Malik,Yaroslav Ilnytskyy,Vladimir Espinosa Angarica,Jinghua Gao,Vorapin Chinchalongporn,Ana-Maria Oproescu,Lakshmy Vasan,Yacine Touahri,Luke Ajay David,Eko Raharjo,Jung-Woong Kim,Wei Wu,Waleed Rahmani,Jennifer Ai-Wen Chan,Igor Kovalchuk,Liliana Attisano,Deborah Kurrasch,Colette Dehay,Anand Swaroop,Diogo S Castro,Jeff Biernaskie,Antonio Del Sol,Carol Schuurmans

Nature protocols 16:1062-1088 PubMed33452502

2021

Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabet Rosàs-Canyelles,Andrew J Modzelewski,Alisha Geldert,Lin He,Amy E Herr

Redox biology 40:101858 PubMed33461096

2021

Reactive oxygen species induce Cys106-mediated anti-parallel HMGB1 dimerization that protects against DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Man Sup Kwak,Woo Joong Rhee,Yong Joon Lee,Hee Sue Kim,Young Hun Kim,Min Kyung Kwon,Jeon-Soo Shin

Brain research bulletin 166:21-28 PubMed33053435

2020

Mechanism of TREM2/DAP12 complex affecting β-amyloid plaque deposition in Alzheimer's disease modeled mice through mediating inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Cui,Jun Qiao,Sha Liu,Ming Wu,Weiwei Gu

View all publications

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Product promise

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