Anti-GFP antibody [E385]
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- What is this?
5
(4 Reviews)
|
(121 Publications)
Anti-GFP antibody [E385] (ab32146) is a rabbit monoclonal antibody detecting GFP in Western Blot.
- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications
- Trusted since 2006
See more GFP antibodies and assay kits
View Alternative Names
Green fluorescent protein, GFP
- WB
Lab
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and dilution buffer : 5% NFDM /TBST.
All lanes:
Western blot - Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution
Lane 1:
HeLa whole cell lysate (negative control)
Lane 2:
HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg
Lane 3:
HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg
Lane 4:
HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
false
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] - (ab129002) staining at 1/10000 dilution.
All lanes:
Western blot - Anti-Patched / PTCH1 antibody [EPR26072-78] (<a href='/en-us/products/primary-antibodies/patched-ptch1-antibody-epr26072-78-ab325234'>ab325234</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 10 µg
Lane 2:
293T transfected with PTCH1 (WT) expression vector containing an EGFP-Myc-tag whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 160-180 kDa,124 kDa,200 kDa
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 35kDa dilution.
All lanes:
Western blot - Anti-Rat Poly PR antibody [PERA534G] (<a href='/en-us/products/primary-antibodies/rat-poly-pr-antibody-pera534g-ab323367'>ab323367</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a EGPF-tag whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a poly (PR)20 expression vector containi a EGFP-tag whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/20000 dilution
Observed band size: 35 kDa,124 kDa
false
Exposure time: 1s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody does not cross-react with human PADI1, PADI3, PADI4 and PADI6.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-PADI2 / PAD2 antibody [EPR26940-85] (<a href='/en-us/products/primary-antibodies/padi2-pad2-antibody-epr26940-85-ab322197'>ab322197</a>) at 1/1000 dilution
Lane 1:
293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human PADI2 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 3:
293T cells transfected with a human PADI1 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 4:
293T cells transfected with a human PADI3 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 5:
293T cells transfected with a human PADI4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 6:
293T cells transfected with a human PADI6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 110 kDa,36 kDa,75 kDa
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody does not cross-react with human NUAK1.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/en-us/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human NUAK1 expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3:
293T cells transfected with a human NUAK2 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 125 kDa,15 kDa,100 kDa
false
Exposure time: 3s
- WB
Unknown
Western blot - Anti-GFP antibody [E385] (AB32146)
All lanes:
Western blot - Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution
Lane 1:
HeLa cells lysate (negative control)
Lane 2:
HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng
Lane 3:
HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng
Lane 4:
HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng
Lane 5:
HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng
Lane 6:
HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng
Lane 7:
Proprietary tag-GFP 0.1mg/ml (positive control)
Predicted band size: 27 kDa
false
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/en-us/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution
Lane 1:
293T cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human GPCR GPRC5D expression vector containing a a GFP-tag, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 33 kDa,15 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The identity of the bands higher than 100 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-AKT (phospho T308) antibody [EPR26565-137] (<a href='/en-us/products/primary-antibodies/akt-phospho-t308-antibody-epr26565-137-ab324741'>ab324741</a>) at 1/5000 dilution
Lane 1:
Untreated 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
293T treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg
Lane 3:
293T transfected with AKT1 (WT) expression vector containing a GFP-tag treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg
Lane 4:
293T transfected with AKT1 (T308A) expression vector containing a GFP-tag treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 60 kDa,36 kDa,86 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-GFP tag antibody (ab32146) staining at 1/1000 dilution. Exposure time : 10 seconds
All lanes:
Western blot - Anti-Vaccinia Virus antibody [EPR28236-80] (<a href='/en-us/products/primary-antibodies/vaccinia-virus-antibody-epr28236-80-ab312854'>ab312854</a>) at 1/1000 dilution
Lane 1:
293T cells transfected with an empty vector containi a GFP tag whole cell lysate at 20 µg
Lane 2:
293T transfected with a Virion membrane protein A21 expression vector containi a GFP tag whole cell lysate at 20 µg
Lane 3:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5:
Human kidney tissue lysate at 20 µg
Lane 6:
Mouse kidney tissue lysate at 20 µg
Lane 7:
Rat kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 46 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody does not cross-react with human PADI1, PADI2, PADI3 and PADI6.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-PADI4 / PAD4 antibody [EPR26687-49] (<a href='/en-us/products/primary-antibodies/padi4-pad4-antibody-epr26687-49-ab315269'>ab315269</a>) at 1/1000 dilution
Lane 1:
293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human PADI4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3:
293T cells transfected with a human PADI1 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 4:
293T cells transfected with a human PADI3 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 5:
293T cells transfected with a human PADI2 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 6:
293T cells transfected with a human PADI6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 75 kDa,36 kDa
false
Exposure time: 6s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-Smt3 antibody [EPR28614-578] (<a href='/en-us/products/primary-antibodies/smt3-antibody-epr28614-578-ab322402'>ab322402</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP-tag, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 16 kDa,37 kDa,36 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/10000 dilution.
In Western blot, Anti-TIM 4 antibody [EPR22304-3] (ab222093) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-HA tag antibody [RM1058] (<a href='/en-us/products/primary-antibodies/ha-tag-antibody-rm1058-ab314237'>ab314237</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate at 20 µg
Lane 2:
293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 78 kDa,36 kDa
false
Exposure time: 37s
- WB
Supplier Data
Western blot - Anti-GFP antibody [E385] (AB32146)
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/10000 dilution.
All lanes:
Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (<a href='/en-us/products/primary-antibodies/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738'>ab317738</a>) at 1/5000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a EGFP tag, whole cell lysate at 2 µg with NFDM/TBST
Lane 2:
293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate 2 at 2 µg with NFDM/TBST
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 40 kDa
false
Exposure time: 180s
Related conjugates and formulations (2)
-
HRP Anti-GFP antibody [E385]
-
Anti-GFP antibody [E385] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-GFP antibody [E385] (ab32146) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB). Trusted by the scientific community
Anti-GFP [E385] (ab32146) was first used in a scientific publication in 2006 and has been cited over 90 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [E385] also available for your convenience: ab32146, HRP - ab190584, Carrier free - ab239807
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (121)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in plant science 16:1590359 PubMed40894500
2025
Applications
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Species
Unspecified reactive species
Nucleic acids research 53: PubMed40650980
2025
Applications
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Species
Unspecified reactive species
aBIOTECH 6:232-248 PubMed40641643
2025
Applications
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Cell death & disease 16:141 PubMed40016178
2025
Applications
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Species
Unspecified reactive species
Nature communications 16:1683 PubMed39956820
2025
Applications
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Species
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The Plant cell 37: PubMed39951393
2025
Applications
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Species
Unspecified reactive species
Molecular therapy. Nucleic acids 36:102442 PubMed39897579
2025
Applications
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Species
Unspecified reactive species
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2416484 PubMed39887931
2025
Applications
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Species
Unspecified reactive species
Acta biochimica et biophysica Sinica 57:805-817 PubMed39574164
2024
Applications
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Species
Unspecified reactive species
Cell death & disease 15:814 PubMed39528487
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com