Anti-GFP antibody [E385] - BSA and Azide free

• WB

Lab

Western blot - Anti-GFP antibody [E385] - BSA and Azide free (AB239807)

This data was developed using ab32146, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GFP antibody [E385] (<a href='/en-us/products/primary-antibodies/gfp-antibody-e385-ab32146'>ab32146</a>) at 1/20000 dilution

Lane 1:

HeLa whole cell lysate (negative control)

Lane 2:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg

Lane 3:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg

Lane 4:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

• WB

Unknown

Western blot - Anti-GFP antibody [E385] - BSA and Azide free (AB239807)

This data was developed using ab32146, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GFP antibody [E385] (<a href='/en-us/products/primary-antibodies/gfp-antibody-e385-ab32146'>ab32146</a>) at 1/20000 dilution

Lane 1:

HeLa cells lysate (negative control)

Lane 2:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng

Lane 3:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng

Lane 4:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng

Lane 5:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng

Lane 6:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng

Lane 7:

Proprietary tag-GFP 0.1mg/ml (positive control)

Predicted band size: 27 kDa

false