Rabbit Recombinant Monoclonal GFP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Tag samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119339.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117)")
![Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunocytochemistry-immunofluorescence-img14347.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98862.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117)")
![Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--flow-cytometry-intracellular-img13159.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (AB236117)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Tag | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Alternative names
Green fluorescent protein, GFP
Recommended products
Rabbit Recombinant Monoclonal GFP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Tag samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14104-89
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 8.82 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236117 is the carrier-free version of Anti-GFP antibody [EPR14104-89] ab183735.
On the basis of low sequence homology, Anti-GFP antibody [EPR14104-89] ab183735 is predicted to show no or limited cross-reactivity to RFP, YFP, BFP, and CFP.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119339.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)
Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse colon tissue (left) and normal mouse colon tissue (right) labeling GFP with Anti-GFP antibody [EPR14104-89] ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFP antibody [EPR14104-89] ab183735).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunocytochemistry-immunofluorescence-img14347.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)")
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)
Immunofluorescent analysis of 4% paraformaldehyde-fixed GFP transfected 293 cells labeling GFP with Anti-GFP antibody [EPR14104-89] ab183735 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFP antibody [EPR14104-89] ab183735).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98862.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)
Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse liver tissue (left) and normal mouse liver tissue (right) labeling GFP with Anti-GFP antibody [EPR14104-89] ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFP antibody [EPR14104-89] ab183735).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117), expandable thumbnail](https://content.abcam.com/products/images/gfp-antibody-epr14104-89-bsa-and-azide-free-ab236117--flow-cytometry-intracellular-img13159.jpg "Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)")
Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104-89] - BSA and Azide free (ab236117)
Intracellular Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP cells labeling GFP with unpurified Anti-GFP antibody [EPR14104-89] ab183735 at 1/200 dilution (10ug/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFP antibody [EPR14104-89] ab183735).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com