Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric)

8 product images

• 

  Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  GFP Western blot staining using chicken Anti-GFP antibody

  Blocking / Diluting buffer and concentration: 5% NFDM/TBST
  
  Exposure time: 3.25 seconds

  All lanes: Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643) at 1/1000 dilution

  Lane 1: Non-transfected 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 2: GFP transfected 293 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/20000 dilution

  Observed band size: 27 kDa

• 

  Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Flow Cytometry (Intracellular) analysis of 293T transfected with GFP tagged Firefly Luciferase over expression vector labeling GFP (right) with ab300643 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti-Chicken IgY H&L (Alexa Fluor® 647, Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175) was used as the secondary antibody at a 1/5000 dilution.  Chicken IgY Isotype control. (left)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with GFP expression vector containing a his tag. (B) Wild type HEK-293T cells labeling GFP with ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on HEK-293T transfected with GFP expression vector containing a his tag (Image: A); No staining on Wild type HEK-293T cells (Image: B). The section was incubated with ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunohistochemical analysis of paraffin-embedded human tonsil labeling GFP with ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on human tonsil. The section was incubated with ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the Leica DS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunohistochemical analysis of paraffin-embedded mouse spleen labeling GFP with ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on mouse spleen. The section was incubated with ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunohistochemical analysis of paraffin-embedded rat spleen labeling GFP with ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on rat spleen. The section was incubated with ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
  
  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 293/GFP (human embryonic kidney epithelial cell) cells labeling GFP with ab300643 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in 293/GFP cells. The nuclear counter stain is DAPI (blue).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (ab300643)

  Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cells) labeling GFP with ab300643 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in HEK-293T cells transfected with a Firefly Luciferase expression vector containing a GFP tag. The nuclear counter stain is DAPI (blue).