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Chicken Recombinant Monoclonal GFP antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Tag samples.

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Images

Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (AB300644), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (AB300644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (AB300644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (AB300644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (AB300644), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Tag
Tested
Tested
Tested
Tested

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

Recommended products

Chicken Recombinant Monoclonal GFP antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Tag samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14104
Purification technique
Affinity purification Thiophilic Resin
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab300644 is the carrier-free version of Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643.

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-GFP antibody [EPR14104] ab183734). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    GFP Western blot staining using chicken Anti-GFP antibody

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643) at 1/1000 dilution

    Lane 1: Non-transfected 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: GFP transfected 293 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (Goat Anti-Chicken IgY H&L (HRP) ab6877) at 1/20000 dilution

    Observed band size: 27 kDa

  • Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Flow Cytometry (Intracellular) analysis of 293T transfected with GFP tagged Firefly Luciferase over expression vector labeling GFP (right) with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti-Chicken IgY H&L (Alexa Fluor® 647, Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175) was used as the secondary antibody at a 1/5000 dilution.  Chicken IgY Isotype control. (left)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with GFP expression vector containing a his tag. (B) Wild type HEK-293T cells labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on HEK-293T transfected with GFP expression vector containing a his tag (Image: A); No staining on Wild type HEK-293T cells (Image: B). The section was incubated with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on human tonsil. The section was incubated with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the Leica DS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on mouse spleen. The section was incubated with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat spleen labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/10000 dilution (0.996 µg/ml) followed by ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. No staining on rat spleen. The section was incubated with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 for 30 mins at room temperature followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 293/GFP (human embryonic kidney epithelial cell) cells labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in 293/GFP cells. The nuclear counter stain is DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) - BSA and Azide free (ab300644)

    This data was developed using Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cells) labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/50 dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in HEK-293T cells transfected with a Firefly Luciferase expression vector containing a GFP tag. The nuclear counter stain is DAPI (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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