Anti-GFP antibody [EPR28018-38] Rabbit monoclonal (ab314656)

Rabbit Recombinant Monoclonal GFP antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP and reacts with Tag samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR28018-38] (AB314656), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt (Intra)	IHC-P	ICC/IF	WB	IP
Tag	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/50	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/30	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information GFP

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

Recommended products

Rabbit Recombinant Monoclonal GFP antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP and reacts with Tag samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28018-38
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR28018-38] (ab314656)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling GFP with ab314656 at 1/50 (10.18 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a empty expression vector containing a GFP tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at 1/1000 (2 ug/mL) dilution.
Western blot - Anti-GFP antibody [EPR28018-38] (ab314656)
Blocking and diluting buffer and concentration: 5% NFDM/TBST,
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GFP antibody [EPR14104] (Anti-GFP antibody [EPR14104] ab183734) staining at 1/5000 dilution.
All lanes: Western blot - Anti-GFP antibody [EPR28018-38] (ab314656) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 7 µg
Lane 2: 293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate at 7 µg
Lane 3: 293T transfected with an empty vector containing a GFP-tag, whole cell lysate at 7 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 26 kDa, 36 kDa
Exposure time: 3s
Immunoprecipitation - Anti-GFP antibody [EPR28018-38] (ab314656)
GFP was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate with ab314656 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314656 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate
Lane 2: ab314656 IP in 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314656 in 293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-GFP antibody [EPR28018-38] (ab314656) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 26 kDa
Exposure time: 6s
Immunoprecipitation - Anti-GFP antibody [EPR28018-38] (ab314656)
GFP was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-tag, whole cell lysate with ab314656 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314656 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-tag, whole cell lysate
Lane 2: ab314656 IP in 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-tag, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314656 in 293T transfected with an empty vector containing a GFP-tag, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-GFP antibody [EPR28018-38] (ab314656) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-tag, whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
Flow Cytometry (Intracellular) - Anti-GFP antibody [EPR28018-38] (ab314656)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a EGFP expression vector containing a EGFP tag (Right) cells labelling GFP with ab314656 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 647 / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR28018-38] (ab314656)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GFP with ab314656 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat spleen. The section was incubated with ab314656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR28018-38] (ab314656)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GFP with ab314656 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse spleen. The section was incubated with ab314656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR28018-38] (ab314656)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling GFP with ab314656 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab314656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR28018-38] (ab314656)
Immunohistochemical analysis of paraffin-embedded (A) 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-tag (B) 293T transfected with an empty vector containing an EGFP-myc-tag (C) 293T transfected with an empty vector containing a myc-His-tag® tissue labeling GFP with ab314656 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on 293T transfected with an empty vector containing a GFP-tag cell pellets (A) and 293T transfected with an empty vector containing an EGFP-myc-tag cell pellets (B). No staining on 293T transfected with an empty vector containing a myc-His-tag cell pellets (C). The section was incubated with ab314656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.