Rabbit Recombinant Monoclonal GFPT2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Recombinant fragment, Rat samples.
Images
![Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--immunoprecipitation-img16133.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316)")
![Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--immunocytochemistry-immunofluorescence-img13985.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316)")
![Flow Cytometry (Intracellular) - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--flow-cytometry-intracellular-img113463.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (AB240316)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Expected | Predicted | Predicted |
| Recombinant fragment | Not recommended | Expected | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
2 products for Alternative Version
Target data
Function
Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
Alternative names
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, Hexosephosphate aminotransferase 2, GFAT 2, GFAT2, GFPT2
Recommended products
Rabbit Recombinant Monoclonal GFPT2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Recombinant fragment, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19095
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240316 is the carrier-free version of Anti-GFPT2 antibody [EPR19095] ab190966.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--immunoprecipitation-img16133.jpg "Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)")
Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)
GFPT2 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate with Anti-GFPT2 antibody [EPR19095] ab190966 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using Anti-GFPT2 antibody [EPR19095] ab190966 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10ug (Input).
Lane 2: Anti-GFPT2 antibody [EPR19095] ab190966 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GFPT2 antibody [EPR19095] ab190966 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFPT2 antibody [EPR19095] ab190966).
All lanes: Immunoprecipitation - Anti-GFPT2 antibody [EPR19095] (Anti-GFPT2 antibody [EPR19095] ab190966)
Predicted band size: 77 kDa
Observed band size: 77 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--immunocytochemistry-immunofluorescence-img13985.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)")
Immunocytochemistry/ Immunofluorescence - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling GFPT2 with Anti-GFPT2 antibody [EPR19095] ab190966 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasm staining on U-87 MG cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-GFPT2 antibody [EPR19095] ab190966 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFPT2 antibody [EPR19095] ab190966).
![Flow Cytometry (Intracellular) - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316), expandable thumbnail](https://content.abcam.com/products/images/gfpt2-antibody-epr19095-bsa-and-azide-free-ab240316--flow-cytometry-intracellular-img113463.jpg "Flow Cytometry (Intracellular) - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)")
Flow Cytometry (Intracellular) - Anti-GFPT2 antibody [EPR19095] - BSA and Azide free (ab240316)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling GFPT2 with Anti-GFPT2 antibody [EPR19095] ab190966 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue).
Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GFPT2 antibody [EPR19095] ab190966).
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